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Browse BarleyBase Experiment Data

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Expression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)
David L Parrott, Montana State University (dparrott@montana.edu)
Experiment design (12 hybridizations)
treatment
•Untreated Control •Empty Virus (no inserted plant sequences) •Rbaal VIGS •Co-silencing (Rbaal + PDS)

VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were com...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)
Accession No: BB108
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•Untreated Control   •Empty Virus (no inserted plant sequences)   •Rbaal VIGS   •Co-silencing (Rbaal + PDS)
Quality Control: biological replicates
Treatment summary:
 treatment  # replicates
 Untreated Control  3
 Empty Virus (no inserted plant sequences)  3
 Rbaal VIGS  3
 Co-silencing (Rbaal + PDS)  3
Total hybridizations: 12
Description: VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were compared between untreated control leaves, virus treated leaves, VIGS-Rbaal knockdown leaves and VIGS-Rbaal/PDS (phytoene desaturase) co-silenced leaves.

Submitted at NCBI GEO via PLEXdb.

Publication: none
Created: 2011-11-15 15:14:10
Last Update: 2011-11-23 13:29:39
Released: 2011-11-23
GEO Accession GSE34102
Submitter: David L Parrott
Name: David L Parrott
Institution: Montana State University
Head of Laboratory: Andreas M Fischer
email(s):
Homepage:

 
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