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Browse BarleyBase Experiment Data

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Developing seeds of M955 low phytic acid barley 7 days after anthesis
David Bowen, University of Idaho (David.Bowen@Pioneer.com)
Experiment design (9 hybridizations)
genotype
•M955 LPA sib-selection •M955 WT sib-selection
year
•2003 •2004

Effect of the low phytic acid mutation on gene expression of developing seeds of M955, a low phytic acid barley genotype with >90% reduction in phy...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Developing seeds of M955 low phytic acid barley 7 days after anthesis
Accession No: BB22
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•M955 LPA sib-selection  •M955 WT sib-selection
year
•2003  •2004
Quality Control: biological replicates
Treatment summary:
 genotype  year  # replicates
 M955 LPA sib-selection  2003  2
 M955 LPA sib-selection  2004  2
 M955 WT sib-selection  2003  3
 M955 WT sib-selection  2004  2
Total hybridizations: 9
Description: Effect of the low phytic acid mutation on gene expression of developing seeds of M955, a low phytic acid barley genotype with >90% reduction in phytic acid in mature seeds. Expression analysis using the Barley1 GeneChip was performed on total RNA from developing seeds (7DAA) of M955 lpa and wt sib-selections. Sib-selections were from BC2 populations of M955 with Harrington as the recurrent parent. Material was grown in field trials in two years 2003 and 2004. In 2003 expression analysis was done from 2 lpa and 3 wt sib-selections, and in 2004 analysis was done from 2 lpa and 2 wt sib-selections.

Submitted at NCBI GEO via PLEXdb.

Publication: 'A Low Phytic Acid Barley Mutation Alters Seed Gene Expression', David E. Bowen, Edward J. Souza, Mary J. Guttieri, Victor Raboy, and Jianming Fu
Crop Sci 2007;47 S-149-S-159
Created: 2007-07-03 09:48:04
Last Update: 2008-02-19 19:49:05
GEO Accession GSE33395
Submitter: David E Bowen
Name: David Bowen
Institution: University of Idaho
Head of Laboratory: Edward Souza
email(s):
Homepage:

 
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