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Mla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions
Rico Caldo, Iowa State University (rpwise@iastate.edu)
Experiment design (108 hybridizations)
genotype
•CI 16137 (Mla1) •CI 16151 (Mla6) •CI 16155 (Mla13)
pathogen isolates
•Bgh 5874 •Bgh K1
time
•0 hai •8 hai •16 hai •20 hai •24 hai •32 hai

A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChi...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Mla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions
Accession No: BB4
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•CI 16137 (Mla1)  •CI 16151 (Mla6)  •CI 16155 (Mla13)
pathogen isolates
•Bgh 5874  •Bgh K1
time
•0 hai  •8 hai  •16 hai  •20 hai  •24 hai  •32 hai
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen isolates  time  # replicates
 CI 16137 (Mla1)  Bgh 5874  0 hai  3
 CI 16137 (Mla1)  Bgh 5874  8 hai  3
 CI 16137 (Mla1)  Bgh 5874  16 hai  3
 CI 16137 (Mla1)  Bgh 5874  20 hai  3
 CI 16137 (Mla1)  Bgh 5874  24 hai  3
 CI 16137 (Mla1)  Bgh 5874  32 hai  3
 CI 16137 (Mla1)  Bgh K1  0 hai  3
 CI 16137 (Mla1)  Bgh K1  8 hai  3
 CI 16137 (Mla1)  Bgh K1  16 hai  3
 CI 16137 (Mla1)  Bgh K1  20 hai  3
 CI 16137 (Mla1)  Bgh K1  24 hai  3
 CI 16137 (Mla1)  Bgh K1  32 hai  3
 CI 16151 (Mla6)  Bgh 5874  0 hai  3
 CI 16151 (Mla6)  Bgh 5874  8 hai  3
 CI 16151 (Mla6)  Bgh 5874  16 hai  3
 CI 16151 (Mla6)  Bgh 5874  20 hai  3
 CI 16151 (Mla6)  Bgh 5874  24 hai  3
 CI 16151 (Mla6)  Bgh 5874  32 hai  3
 CI 16151 (Mla6)  Bgh K1  0 hai  3
 CI 16151 (Mla6)  Bgh K1  8 hai  3
 CI 16151 (Mla6)  Bgh K1  16 hai  3
 CI 16151 (Mla6)  Bgh K1  20 hai  3
 CI 16151 (Mla6)  Bgh K1  24 hai  3
 CI 16151 (Mla6)  Bgh K1  32 hai  3
 CI 16155 (Mla13)  Bgh 5874  0 hai  3
 CI 16155 (Mla13)  Bgh 5874  8 hai  3
 CI 16155 (Mla13)  Bgh 5874  16 hai  3
 CI 16155 (Mla13)  Bgh 5874  20 hai  3
 CI 16155 (Mla13)  Bgh 5874  24 hai  3
 CI 16155 (Mla13)  Bgh 5874  32 hai  3
 CI 16155 (Mla13)  Bgh K1  0 hai  3
 CI 16155 (Mla13)  Bgh K1  8 hai  3
 CI 16155 (Mla13)  Bgh K1  16 hai  3
 CI 16155 (Mla13)  Bgh K1  20 hai  3
 CI 16155 (Mla13)  Bgh K1  24 hai  3
 CI 16155 (Mla13)  Bgh K1  32 hai  3
Total hybridizations: 108
Description: A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-dependent vs. -independent interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13).

Submitted at NCBI GEO via PLEXdb.

Publication: 'Transcript profiling in host-pathogen interactions', Wise RP, Moscou MJ, Bogdanove AJ, Whitham SA
2007. Annual Review of Phytopathology 45: 329-369.
pubmed: 17480183
'Estimating the number of true null hypotheses from a histogram of p-values', Nettleton D, Hwang JTG, Caldo RA, Wise RP
2006. Journal of Agricultural, Biological, and Environmental Statistics 11: 337-356.'Stage-specific suppression of basal defense discriminates barley plants containing fast- and delayed-acting Mla powdery mildew resistance alleles', Caldo RA, Nettleton D, Peng J, Wise RP
Molecular Plant-Microbe Interactions. 2006 Sep;19(9):939-47
pubmed: 16941898
'Interaction-Dependent Gene Expression in Mla-Specified Response to Barley Powdery Mildew', Rico A. Caldo, Dan Nettleton and Roger P. Wise
Plant Cell. 16:2514-2528 (2004)
pubmed: 15319481
Created: 2004-10-28 13:24:45
Last Update: 2011-10-14 09:44:24
GEO Accession GSE33396
Submitter: Rico A. Caldo
Name: Rico Caldo
Institution: Iowa State University
Head of Laboratory: Roger P Wise
email(s):
Homepage:

 
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