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Browse BarleyBase Experiment Data

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Carbohydrate accumulation in barley leaves leads to senescence and protease gene upregulation
David Parrott, Montana State University (dparrott@montana.edu)
Experiment design (21 hybridizations)
time
•0 Day •4 Day •8 Day
Treated or untreated
•untreated control •girdled •shift-girdled control

Sugar accumulation was induced in mature barley (Hordeum vulgare L.) leaves by the interruption of sieve tubes at the base of the leaf lamina by â€...[complete overview]

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Experiment Name: Carbohydrate accumulation in barley leaves leads to senescence and protease gene upregulation
Accession No: BB50
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•0 Day  •4 Day  •8 Day
Treated or untreated
•untreated control  •girdled  •shift-girdled control
Quality Control: biological replicates
Treatment summary:
 time  Treated or untreated  # replicates
 0 Day  untreated control  3
 4 Day  untreated control  3
 4 Day  girdled  3
 4 Day  shift-girdled control  3
 8 Day  untreated control  3
 8 Day  girdled  3
 8 Day  shift-girdled control  3
Total hybridizations: 21
Description: Sugar accumulation was induced in mature barley (Hordeum vulgare L.) leaves by the interruption of sieve tubes at the base of the leaf lamina by “steam-girdling”. Using the Affymetrix Barley1 genome array we determined up-regulated protease genes in girdled leaves as compared to untreated control leaves. Additionally, we examine in greater detail leaf carbohydrate accumulation, Rubisco degradation and determine biological processes affected by carbohydrate accumulation in barley leaves. Using this functional genomic approach to study degradation of major leaf proteins may considerably improve our understanding of whole-plant nitrogen recycling.
Publication: 'Steam-girdling of barley (Hordeum vulgare) leaves leads to carbohydrate accumulation and accelerated leaf senescence, facilitating transcriptomic analysis of senescence-associated genes', Parrott DL, McInnerney K, Feller U, Fischer AM
2007 New Phytologist 176: 56–69
pubmed: 17803641
Created: 2006-10-12 17:11:55
Last Update: 2008-08-05 11:40:22
Submitter: David L Parrott
Name: David Parrott
Institution: Montana State University
Head of Laboratory: Andreas Fischer
email(s):
Homepage:

 
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