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Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation
Aravind Jukanti, MSU-Bozeman (jukanti5@yahoo.com)
Experiment design (24 hybridizations)
developmental stage
•14 days past anthesis •21 days past anthesis
genotype
•Karl •10-11 (NIL with high grain protein locus)
tissue type
•Leaves •Kernels

Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation
Accession No: BB53
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
developmental stage
•14 days past anthesis  •21 days past anthesis
genotype
•Karl  •10-11 (NIL with high grain protein locus)
tissue type
•Leaves  •Kernels
Quality Control: biological replicates
Treatment summary:
 developmental stage  genotype  tissue type  # replicates
 14 days past anthesis  Karl  Leaves  3
 14 days past anthesis  Karl  Kernels  3
 14 days past anthesis  10-11 (NIL with high grain protein locus)  Leaves  3
 14 days past anthesis  10-11 (NIL with high grain protein locus)  Kernels  3
 21 days past anthesis  Karl  Leaves  3
 21 days past anthesis  Karl  Kernels  3
 21 days past anthesis  10-11 (NIL with high grain protein locus)  Leaves  3
 21 days past anthesis  10-11 (NIL with high grain protein locus)  Kernels  3
Total hybridizations: 24
Description: Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety with its near-isogenic line 10_11(has high grain protein locus, chromosome 6)using Barley1 22k affymetrix chip.

Submitted at NCBI GEO via PLEXdb.

Publication: 'Comparative transcriptome profiling of near-isogenic barley (Hordeum vulgare) lines differing in the allelic state of a major grain protein content locus identifies genes with possible roles in leaf senescence and nitrogen reallocation.', Aravind K. Jukanti, Nancy M. Heidlebaugh, David L. Parrott, Isabelle A. Fischer, Kate McInnerney and Andreas M. Fischer
New Phytologist 177(2):333-349 (Jan 2008).
pubmed: 18028296
Created: 2006-12-18 16:35:53
Last Update: 2008-08-05 11:41:27
GEO Accession GSE33413
Submitter: Aravind K Jukanti
Name: Aravind Jukanti
Institution: MSU-Bozeman
Head of Laboratory:
email(s):
Homepage:

 
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