Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse BarleyBase Experiment Data

Choose an experiment:
Transcriptome analysis of wild barley (H. vulgare ssp spontaneum) after pathogen inoculation
Ben Millett, University of Minnesota (mill1964@umn.edu, muehl003@umn.edu)
Experiment design (48 hybridizations)
treated or untreated
•C. sativus (spot blotch) •water control •P. hordei (leaf rust) •oil control
time
•12 hrs •24 hrs •36 hrs •48 hrs

Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was submitted to 4 experimental treatments (C. sativus (spot blotch), P. hordei (le...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Transcriptome analysis of wild barley (H. vulgare ssp spontaneum) after pathogen inoculation
Accession No: BB61
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
treated or untreated
•C. sativus (spot blotch)   •water control   •P. hordei (leaf rust)   •oil control
time
•12 hrs  •24 hrs  •36 hrs  •48 hrs
Quality Control: biological replicates
Treatment summary:
 treated or untreated  time  # replicates
 C. sativus (spot blotch)  12 hrs  3
 C. sativus (spot blotch)  24 hrs  3
 C. sativus (spot blotch)  36 hrs  3
 C. sativus (spot blotch)  48 hrs  3
 water control  12 hrs  3
 water control  24 hrs  3
 water control  36 hrs  3
 water control  48 hrs  3
 P. hordei (leaf rust)  12 hrs  3
 P. hordei (leaf rust)  24 hrs  3
 P. hordei (leaf rust)  36 hrs  3
 P. hordei (leaf rust)  48 hrs  3
 oil control  12 hrs  3
 oil control  24 hrs  3
 oil control  36 hrs  3
 oil control  48 hrs  3
Total hybridizations: 48
Description: Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was
submitted to 4 experimental treatments (C. sativus (spot blotch), P.
hordei (leaf rust), and water and oil controls) to examine gene
transcription differences triggered by biotrophic and hemi-biotrophic
pathogens. Inoculated plants were arranged in a split plot design.
Samples were collected at 12, 24, 36, & 48 hours after inoculation. A
total of 48 samples (4 treatments x 4 time points x 3 replicates) were
subjected to GeneChip analysis.

Made public: 2009-12-02

Submitted at NCBI GEO via PLEXdb.

Publication: 'Wild barley expresses distinct sets of genes in response to pathogens of different trophic lifestyles.', Millett, B.P., Y. Xiong, S. Dahl, B.J. Steffenson, G.J. Muehlbauer.
Physiol. & Mol. Plant Path. (2009)
Created: 2008-06-12 11:22:13
Last Update: 2008-07-11 15:22:33
Released: 2009-12-02
GEO Accession GSE33401
Submitter: Ben Millett
Name: Ben Millett
Institution: University of Minnesota
Head of Laboratory: Gary Muehlbauer
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters