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Browse BarleyBase Experiment Data

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Transcriptome analysis of wild barley (H. vulgare ssp spontaneum) after pathogen inoculation
Ben Millett, University of Minnesota (mill1964@umn.edu, muehl003@umn.edu)
Experiment design (48 hybridizations)
treated or untreated
•C. sativus (spot blotch) •water control •P. hordei (leaf rust) •oil control
time
•12 hrs •24 hrs •36 hrs •48 hrs

Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was submitted to 4 experimental treatments (C. sativus (spot blotch), P. hordei (le...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptome analysis of wild barley (H. vulgare ssp spontaneum) after pathogen inoculation
Accession No: BB61
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
treated or untreated
•C. sativus (spot blotch)   •water control   •P. hordei (leaf rust)   •oil control
time
•12 hrs  •24 hrs  •36 hrs  •48 hrs
Quality Control: biological replicates
Treatment summary:
 treated or untreated  time  # replicates
 C. sativus (spot blotch)  12 hrs  3
 C. sativus (spot blotch)  24 hrs  3
 C. sativus (spot blotch)  36 hrs  3
 C. sativus (spot blotch)  48 hrs  3
 water control  12 hrs  3
 water control  24 hrs  3
 water control  36 hrs  3
 water control  48 hrs  3
 P. hordei (leaf rust)  12 hrs  3
 P. hordei (leaf rust)  24 hrs  3
 P. hordei (leaf rust)  36 hrs  3
 P. hordei (leaf rust)  48 hrs  3
 oil control  12 hrs  3
 oil control  24 hrs  3
 oil control  36 hrs  3
 oil control  48 hrs  3
Total hybridizations: 48
Description: Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was
submitted to 4 experimental treatments (C. sativus (spot blotch), P.
hordei (leaf rust), and water and oil controls) to examine gene
transcription differences triggered by biotrophic and hemi-biotrophic
pathogens. Inoculated plants were arranged in a split plot design.
Samples were collected at 12, 24, 36, & 48 hours after inoculation. A
total of 48 samples (4 treatments x 4 time points x 3 replicates) were
subjected to GeneChip analysis.

Made public: 2009-12-02

Submitted at NCBI GEO via PLEXdb.
Publication: 'Wild barley expresses distinct sets of genes in response to pathogens of different trophic lifestyles.', Millett, B.P., Y. Xiong, S. Dahl, B.J. Steffenson, G.J. Muehlbauer.
Physiol. & Mol. Plant Path. (2009)
Created: 2008-06-12 11:22:13
Last Update: 2008-07-11 15:22:33
Released: 2009-12-02
GEO Accession GSE33401
Submitter: Ben Millett
Experimenter:
Name: Ben Millett
Institution: University of Minnesota
Head of Laboratory: Gary Muehlbauer
email(s):
mill1964@umn.edu, muehl003@umn.edu
Homepage:

 
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