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Browse BarleyBase Experiment Data

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Late response to boron toxicity in barley leaves
Mehmet Tufan OZ, Middle East Technical University, Department of Biological Sciences, Plant Biotechnology R&D Labs (oztufan@metu.edu.tr)
Experiment design (9 hybridizations)
treated or untreated
•Control (C) •5 mM B(OH)3 treatment (5B) •10 mM B(OH)3 treatment (10B)

Barley1 GeneChip was used to investigate transcriptome changes associated with boron (B) toxicity in a sensitive barley cultivar (Hordeum vulgare L...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Late response to boron toxicity in barley leaves
Accession No: BB63
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
treated or untreated
•Control (C)   •5 mM B(OH)3 treatment (5B)   •10 mM B(OH)3 treatment (10B)
Quality Control: biological replicates
Treatment summary:
 treated or untreated  # replicates
 Control (C)  3
 5 mM B(OH)3 treatment (5B)  3
 10 mM B(OH)3 treatment (10B)  3
Total hybridizations: 9
Description: Barley1 GeneChip was used to investigate transcriptome changes associated with boron (B) toxicity in a sensitive barley cultivar (Hordeum vulgare L. cv. Hamidiye). Eight day old aseptically grown seedlings were subjected to 5 or 10 mM boric acid (B(OH)3) treatments for 5 days and expression profiles were determined with DNA microarrays using total RNA from leaf tissues. Surface sterilized seeds were aseptically germinated and grown on half strength Hoagland’s solution (Hoagland and Arnon, 1950) (pH 5.8) solidified with Phytagel® for 8 days at 23±2ºC with 16 h light (400 µmol m–2 s–1) and 8 h dark photo-cycle with 70% relative humidity. Seedlings were transferred to sterile hydroponic cultures for B treatment, which was applied immediately after transfer as half strength Hoagland’s solution containing either 5 mM B(OH)3 (5B) or 10 mM B(OH)3 (10B) for another 5 days under the same physical conditions. Control (C) groups were transferred to half strength Hoagland’s solution without extra B(OH)3.
Publication: 'Microarray Analysis of Late Response to Boron Toxicity in Barley (Hordeum vulgare L.) Leaves', z MT, Y?lmaz R, Eyido?an F, de Graaff L, Ycel M, ktem HA ? Mehmet Tufan OZ, Remziye YILMAZ, Fusun EYIDOGAN, Leo de GRAAFF, Meral YUCEL, 2, Huseyin Avni OKTEM
Turkish Journal of Agriculture and Forestry, 33: 191-202, 2009
Created: 2008-11-10 17:14:24
Last Update: 2009-01-12 21:18:31
GEO Accession GSE14521
Submitter: tufan mehmet oz
Name: Mehmet Tufan OZ
Institution: Middle East Technical University, Department of Biological Sciences, Plant Biotechnology R&D Labs
Head of Laboratory: Prof. Dr. Huseyin Avni OKTEM & Prof. Dr. Meral YUCEL, haoktem@metu.edu.tr, meraly@metu.edu.tr
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