Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse BarleyBase Experiment Data

Choose an experiment:
Genetic regulation of gene expression of barley in response to stem rust (Pgt isolate TTKS)
Roger P. Wise, Iowa State University (rpwise@iastate.edu)
Experiment design (166 hybridizations)
genetic line
•Q21861 •SM89010 •QxSM001 •QxSM002 •QxSM003 •QxSM005 •QxSM008 •QxSM010 •QxSM011 •QxSM013 •QxSM014 •QxSM015 •QxSM016 •QxSM017 •QxSM018 •QxSM019 •QxSM023 •QxSM026 •QxSM028 •QxSM029 •QxSM030 •QxSM031 •QxSM033 •QxSM034 •QxSM035 •QxSM036 •QxSM037 •QxSM038 •QxSM039 •QxSM041 •QxSM042 •QxSM043 •QxSM045 •QxSM046 •QxSM047 •QxSM048 •QxSM049 •QxSM050 •QxSM052 •QxSM054 •QxSM055 •QxSM056 •QxSM057 •QxSM058 •QxSM059 •QxSM060 •QxSM061 •QxSM062 •QxSM063 •QxSM065 •QxSM066 •QxSM067 •QxSM068 •QxSM069 •QxSM070 •QxSM073 •QxSM074 •QxSM075 •QxSM076 •QxSM077 •QxSM078 •QxSM079 •QxSM080 •QxSM082 •QxSM083 •QxSM084 •QxSM085 •QxSM086 •QxSM087 •QxSM088 •QxSM089 •QxSM090 •QxSM091 •QxSM093 •QxSM094 •QxSM095 •QxSM097
treated or untreated
•Inoculated - Pgt TTKS •Mock

The QxSM doubled-haploid mapping population was generated from a single Q21861 x SM89010 F1 plant (Borovkova et al. 1995; Steffenson et al. 1995). ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Genetic regulation of gene expression of barley in response to stem rust (Pgt isolate TTKS)
Accession No: BB64
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•Q21861   •SM89010   •QxSM001   •QxSM002   •QxSM003   •QxSM005   •QxSM008   •QxSM010   •QxSM011   •QxSM013   •QxSM014   •QxSM015   •QxSM016   •QxSM017   •QxSM018   •QxSM019   •QxSM023   •QxSM026   •QxSM028   •QxSM029   •QxSM030   •QxSM031   •QxSM033   •QxSM034   •QxSM035   •QxSM036   •QxSM037   •QxSM038   •QxSM039   •QxSM041   •QxSM042   •QxSM043   •QxSM045   •QxSM046   •QxSM047   •QxSM048   •QxSM049   •QxSM050   •QxSM052   •QxSM054   •QxSM055   •QxSM056   •QxSM057   •QxSM058   •QxSM059   •QxSM060   •QxSM061   •QxSM062   •QxSM063   •QxSM065   •QxSM066   •QxSM067   •QxSM068   •QxSM069   •QxSM070   •QxSM073   •QxSM074   •QxSM075   •QxSM076   •QxSM077   •QxSM078   •QxSM079   •QxSM080   •QxSM082   •QxSM083   •QxSM084   •QxSM085   •QxSM086   •QxSM087   •QxSM088   •QxSM089   •QxSM090   •QxSM091   •QxSM093   •QxSM094   •QxSM095   •QxSM097
treated or untreated
•Inoculated - Pgt TTKS   •Mock
Quality Control: biological replicates
Treatment summary:
 genetic line  treated or untreated  # replicates
 Q21861  Inoculated - Pgt TTKS  4
 Q21861  Mock  4
 SM89010  Inoculated - Pgt TTKS  4
 SM89010  Mock  4
 QxSM001  Inoculated - Pgt TTKS  1
 QxSM001  Mock  1
 QxSM002  Inoculated - Pgt TTKS  1
 QxSM002  Mock  1
 QxSM003  Inoculated - Pgt TTKS  1
 QxSM003  Mock  1
 QxSM005  Inoculated - Pgt TTKS  1
 QxSM005  Mock  1
 QxSM008  Inoculated - Pgt TTKS  1
 QxSM008  Mock  1
 QxSM010  Inoculated - Pgt TTKS  1
 QxSM010  Mock  1
 QxSM011  Inoculated - Pgt TTKS  1
 QxSM011  Mock  1
 QxSM013  Inoculated - Pgt TTKS  1
 QxSM013  Mock  1
 QxSM014  Inoculated - Pgt TTKS  1
 QxSM014  Mock  1
 QxSM015  Inoculated - Pgt TTKS  1
 QxSM015  Mock  1
 QxSM016  Inoculated - Pgt TTKS  1
 QxSM016  Mock  1
 QxSM017  Inoculated - Pgt TTKS  1
 QxSM017  Mock  1
 QxSM018  Inoculated - Pgt TTKS  1
 QxSM018  Mock  1
 QxSM019  Inoculated - Pgt TTKS  1
 QxSM019  Mock  1
 QxSM023  Inoculated - Pgt TTKS  1
 QxSM023  Mock  1
 QxSM026  Inoculated - Pgt TTKS  1
 QxSM026  Mock  1
 QxSM028  Inoculated - Pgt TTKS  1
 QxSM028  Mock  1
 QxSM029  Inoculated - Pgt TTKS  1
 QxSM029  Mock  1
 QxSM030  Inoculated - Pgt TTKS  1
 QxSM030  Mock  1
 QxSM031  Inoculated - Pgt TTKS  1
 QxSM031  Mock  1
 QxSM033  Inoculated - Pgt TTKS  1
 QxSM033  Mock  1
 QxSM034  Inoculated - Pgt TTKS  1
 QxSM034  Mock  1
 QxSM035  Inoculated - Pgt TTKS  1
 QxSM035  Mock  1
 QxSM036  Inoculated - Pgt TTKS  1
 QxSM036  Mock  1
 QxSM037  Inoculated - Pgt TTKS  1
 QxSM037  Mock  1
 QxSM038  Inoculated - Pgt TTKS  1
 QxSM038  Mock  1
 QxSM039  Inoculated - Pgt TTKS  1
 QxSM039  Mock  1
 QxSM041  Inoculated - Pgt TTKS  1
 QxSM041  Mock  1
 QxSM042  Inoculated - Pgt TTKS  1
 QxSM042  Mock  1
 QxSM043  Inoculated - Pgt TTKS  1
 QxSM043  Mock  1
 QxSM045  Inoculated - Pgt TTKS  1
 QxSM045  Mock  1
 QxSM046  Inoculated - Pgt TTKS  1
 QxSM046  Mock  1
 QxSM047  Inoculated - Pgt TTKS  1
 QxSM047  Mock  1
 QxSM048  Inoculated - Pgt TTKS  1
 QxSM048  Mock  1
 QxSM049  Inoculated - Pgt TTKS  1
 QxSM049  Mock  1
 QxSM050  Inoculated - Pgt TTKS  1
 QxSM050  Mock  1
 QxSM052  Inoculated - Pgt TTKS  1
 QxSM052  Mock  1
 QxSM054  Inoculated - Pgt TTKS  1
 QxSM054  Mock  1
 QxSM055  Inoculated - Pgt TTKS  1
 QxSM055  Mock  1
 QxSM056  Inoculated - Pgt TTKS  1
 QxSM056  Mock  1
 QxSM057  Inoculated - Pgt TTKS  1
 QxSM057  Mock  1
 QxSM058  Inoculated - Pgt TTKS  1
 QxSM058  Mock  1
 QxSM059  Inoculated - Pgt TTKS  1
 QxSM059  Mock  1
 QxSM060  Inoculated - Pgt TTKS  1
 QxSM060  Mock  1
 QxSM061  Inoculated - Pgt TTKS  1
 QxSM061  Mock  1
 QxSM062  Inoculated - Pgt TTKS  1
 QxSM062  Mock  1
 QxSM063  Inoculated - Pgt TTKS  1
 QxSM063  Mock  1
 QxSM065  Inoculated - Pgt TTKS  1
 QxSM065  Mock  1
 QxSM066  Inoculated - Pgt TTKS  1
 QxSM066  Mock  1
 QxSM067  Inoculated - Pgt TTKS  1
 QxSM067  Mock  1
 QxSM068  Inoculated - Pgt TTKS  1
 QxSM068  Mock  1
 QxSM069  Inoculated - Pgt TTKS  1
 QxSM069  Mock  1
 QxSM070  Inoculated - Pgt TTKS  1
 QxSM070  Mock  1
 QxSM073  Inoculated - Pgt TTKS  1
 QxSM073  Mock  1
 QxSM074  Inoculated - Pgt TTKS  1
 QxSM074  Mock  1
 QxSM075  Inoculated - Pgt TTKS  1
 QxSM075  Mock  1
 QxSM076  Inoculated - Pgt TTKS  1
 QxSM076  Mock  1
 QxSM077  Inoculated - Pgt TTKS  1
 QxSM077  Mock  1
 QxSM078  Inoculated - Pgt TTKS  1
 QxSM078  Mock  1
 QxSM079  Inoculated - Pgt TTKS  1
 QxSM079  Mock  1
 QxSM080  Inoculated - Pgt TTKS  1
 QxSM080  Mock  1
 QxSM082  Inoculated - Pgt TTKS  1
 QxSM082  Mock  1
 QxSM083  Inoculated - Pgt TTKS  1
 QxSM083  Mock  1
 QxSM084  Inoculated - Pgt TTKS  1
 QxSM084  Mock  1
 QxSM085  Inoculated - Pgt TTKS  1
 QxSM085  Mock  1
 QxSM086  Inoculated - Pgt TTKS  1
 QxSM086  Mock  1
 QxSM087  Inoculated - Pgt TTKS  1
 QxSM087  Mock  1
 QxSM088  Inoculated - Pgt TTKS  1
 QxSM088  Mock  1
 QxSM089  Inoculated - Pgt TTKS  1
 QxSM089  Mock  1
 QxSM090  Inoculated - Pgt TTKS  1
 QxSM090  Mock  1
 QxSM091  Inoculated - Pgt TTKS  1
 QxSM091  Mock  1
 QxSM093  Inoculated - Pgt TTKS  1
 QxSM093  Mock  1
 QxSM094  Inoculated - Pgt TTKS  1
 QxSM094  Mock  1
 QxSM095  Inoculated - Pgt TTKS  1
 QxSM095  Mock  1
 QxSM097  Inoculated - Pgt TTKS  1
 QxSM097  Mock  1
Total hybridizations: 166
Description: The QxSM doubled-haploid mapping population was generated from a single Q21861 x SM89010 F1 plant (Borovkova et al. 1995; Steffenson et al. 1995). Four flats (each flat contained 75 DH lines + 4 replicates of each parent = 81 cones/flat) were grown in a completely randomized design at the ARS Cereal Disease Lab, University of Minnesota, St. Paul. The four flats were divided into two replicates of two flats each. Nine days after sowing, one flat of each replicate was inoculated (INOC) with TTKS urediniospores were suspended in Soltrol oil with an inoculum weight of 0.25 mg per flat and the other was mock-inoculated (MOCK). Each (MOCK and INOC) replicate was incubated in its own dew chamber overnight. After inoculation, replicates were placed in separate mist chambers for 16 hours in the dark, followed by lights for 5 hours, and then moved to the greenhouse for 2 hours. The growth stage of barley was first leaf unfolded (PO:0007094) and five seedlings were harvested and placed in liquid nitrogen for each line in the population within a 1.5 hour period at 24 hours after inoculation (hai).

Note: An error was discovered in the original experiment upload: The CEL files were switched in both INOC and MOCK for QSM039 and QSM078. The files have been corrected (2010/10/27) and the data renormalized.

Submitted at NCBI GEO via PLEXdb
Publication: 'Quantitative and Qualitative Stem Rust Resistance Factors in Barley Are Associated with Transcriptional Suppression of Defense Regulons.', Moscou MJ, Lauter N, Steffenson B, Wise RP
PLoS Genet 7(7): e1002208. doi:10.1371/journal.pgen.1002208
Created: 2009-01-23 13:55:49
Last Update: 2009-02-11 13:31:20
Released: 2010-11-15
GEO Accession GSE20416
Submitter: Roger P Wise
Name: Roger P. Wise
Institution: Iowa State University
Head of Laboratory: Roger Wise
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters