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Browse BarleyBase Experiment Data

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Blumeria graminis fsp hordei effects on translation initiation in compatible and incompatible barley.
Jackson Moeller, Iowa State University (jacksonr@iastate.edu, swhitham@iastate.edu)
Experiment design (24 hybridizations)
genotype
•CI 16151 (Mla6) •CI 16151 m9472 (mla6)
pathogen infection
•inoculated with Bgh isolate 5874 (AVRa6) •non-inoculated
RNA fraction
•polysomal RNA •total RNA

The goal of this experiment was to examine the effects of Blumeria gramins f. sp. hordei (Bgh) on the host process of translation initiation in bot...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Blumeria graminis fsp hordei effects on translation initiation in compatible and incompatible barley.
Accession No: BB72
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•CI 16151 (Mla6)   •CI 16151 m9472 (mla6)
pathogen infection
•inoculated with Bgh isolate 5874 (AVRa6)   •non-inoculated
RNA fraction
•polysomal RNA   •total RNA
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen infection  RNA fraction  # replicates
 CI 16151 (Mla6)  inoculated with Bgh isolate 5874 (AVRa6)  polysomal RNA  3
 CI 16151 (Mla6)  inoculated with Bgh isolate 5874 (AVRa6)  total RNA  3
 CI 16151 (Mla6)  non-inoculated  polysomal RNA  3
 CI 16151 (Mla6)  non-inoculated  total RNA  3
 CI 16151 m9472 (mla6)  inoculated with Bgh isolate 5874 (AVRa6)  polysomal RNA  3
 CI 16151 m9472 (mla6)  inoculated with Bgh isolate 5874 (AVRa6)  total RNA  3
 CI 16151 m9472 (mla6)  non-inoculated  polysomal RNA  3
 CI 16151 m9472 (mla6)  non-inoculated  total RNA  3
Total hybridizations: 24
Description: The goal of this experiment was to examine the effects of Blumeria gramins f. sp. hordei (Bgh) on the host process of translation initiation in both incompatible and compatible interactions with Hordeum vulgare. This was done by using Bgh isolate 5874 (AVRa6) on the resistant host CI 16151 (Mla6) and fast-neutron derived, loss-of-function mutant m9472 (mla6). Plants were grown 7 days in the greenhouse and then inoculated or non-inoculated and transferred to an 18°C growth chamber with an 8 hr dark, 16 hr light photoperiod. At 32 hours after infection the tissue was harvested and immediately frozen in liquid nitrogen. From the same tissue, total RNA was isolated using a Trizol method, while polysomal RNA was isolated by sucrose density centrifugation. Three biological replications were conducted.

Submitted at NCBI GEO via PLEXdb
Publication: none
Created: 2009-04-29 14:53:43
Last Update: 2009-05-01 15:25:40
Released: 2011-03-11
GEO Accession GSE20279
Submitter: Jackson R Moeller
Name: Jackson Moeller
Institution: Iowa State University
Head of Laboratory: Steve Whitham
email(s):
Homepage:

 
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