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Browse BarleyBase Experiment Data

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Comparative transcriptional profiling of organs of the barley spike
Tilahun Abebe, University of Northern Iowa (Tilahun.Abebe@uni.edu)
Experiment design (12 hybridizations)
tissue type name
•Lemma •Palea •Awn •Seed

Barley plants (Hordeum vulgare L.) cv. Morex were grown in a controlled environment chamber at 22°C/18°C day/night temperatures, 60% RH and a 16 ...[complete overview]

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Experiment Name: Comparative transcriptional profiling of organs of the barley spike
Accession No: BB73
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
tissue type name
•Lemma   •Palea   •Awn   •Seed
Quality Control: biological replicates
Treatment summary:
 tissue type name  # replicates
 Lemma  3
 Palea  3
 Awn  3
 Seed  3
Total hybridizations: 12
Description: Barley plants (Hordeum vulgare L.) cv. Morex were grown in a controlled environment chamber at 22°C/18°C day/night temperatures, 60% RH and a 16 h photoperiod. Plants were grown in 20 cm x 25 cm pots and fertilized using Osmocote® slow release fertilizer. Lemma, palea, awn and seed were collected at grain-filling stage (Zadok scale 83) to compare differences in gene expression. For each organ three biological replications were collected on three different dates. On each of the three dates, one replication of each organ was collected from plants grown in separate pots using a randomized complete block design (RCBD), which includes organ type as the treatment effect and date of sample collection (replication) as the block. To get enough tissue for RNA extraction, each replication for each organ was collected from four plants in the same pot. RNA from each replicate sample was hybridized to individual Barley1 GeneChips.

PLEXdb Curator's Note:
This experiment has already been submitted at NCBI-GEO by the submitter.
Publication: 'Comparative Transcriptional Profiling Established the Awn as the Major Photosynthetic Organ of the Barley Spike While the Lemma and the Palea Primarily Protect the Seed', Tilahun Abebe, Roger P. Wise, and Ronald W. Skadsen.
Plant Genome 2:247-259 (2009)
Created: 2009-06-15 17:24:40
Last Update: 2009-06-16 09:43:38
GEO Accession GSE16754
Submitter: Tilahun Abebe
Name: Tilahun Abebe
Institution: University of Northern Iowa
Head of Laboratory: Tilahun Abebe
email(s):
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