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Browse BarleyBase Experiment Data

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Expression data from malting barley seeds
Nora,L,Lapitan, Colorado State University, Fort Collins, CO (Nora.Lapitan@ColoState.EDU)
Experiment design (22 hybridizations)
genotype
•Morex •Legacy •Merit •Harrington
treatment
•ungerminated •steeped •1d •4d •kilned

This experiment has been imported by PLEXdb from NCBI GEO (GSE11200)

Series_summary: Malting is seed germination under strictly control...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from malting barley seeds
Accession No: BB87
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Morex   •Legacy   •Merit   •Harrington
treatment
•ungerminated   •steeped   •1d   •4d   •kilned
Quality Control: biological replicates
Treatment summary:
 genotype  treatment  # replicates
 Morex  ungerminated  2
 Morex  steeped  2
 Morex  1d  2
 Morex  4d  2
 Morex  kilned  2
 Legacy  ungerminated  0
 Legacy  steeped  0
 Legacy  1d  2
 Legacy  4d  2
 Legacy  kilned  0
 Merit  ungerminated  0
 Merit  steeped  0
 Merit  1d  2
 Merit  4d  3
 Merit  kilned  0
 Harrington  ungerminated  0
 Harrington  steeped  0
 Harrington  1d  1
 Harrington  4d  2
 Harrington  kilned  0
Total hybridizations: 22
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE11200)

Series_summary:
Malting is seed germination under strictly controlled environmental conditions. Malting quality is a complex phenotype that combines a large number of interrelated components, each of which shows complex inheritance. Currently, only a few genes involved in determining malting quality have been characterized. This study combined transcript profiling with phenotypic correlations to identify candidate genes for malting quality.
We used the Barley1 GeneChipĀ® array to identify differentially expressed genes in four malting stages relative to dry seed in the barley variety Morex, and to identify differentially expressed genes among four barley varieties.
Keywords: time course and genotype differences

Series_overall_design:
Four malting barley cultivars were micromalted: Morex and Legacy (6-row), Merit and Harrington (2-row). Two to three batches of micromalting (biological replications) were performed. For each micromalting experiment, 20 g samples were collected for Morex at four stages: steeping, 24 h germination (day 1), 93 h germination (day 4), and finished malt after kilning was completed. Ungerminated, dry seed of Morex was used as reference sample. In another experiment, seeds of Legacy, Harrington, and Merit were micromalted and 20 g samples were collected for each variety during day 1 and day 4 germination stages. Expression profiles were compared among the four cultivars separately for day 1 and day 4.
Publication: 'Differentially expressed genes during malting and correlation with malting quality phenotypes in barley (Hordeum vulgare L.).', Lapitan NL, Hess A, Cooper B, Botha AM et al.
Theor Appl Genet 2009 Mar;118(5):937-52.
pubmed: 19132335
Created: 2009-07-24 13:32:56
Last Update: 2009-07-24 13:53:55
GEO Accession GSE11200
Submitter: PLEXdb Curator
Name: Nora,L,Lapitan
Institution: Colorado State University, Fort Collins, CO
Head of Laboratory: --
email(s):
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