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Browse BarleyBase Experiment Data

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Gene expression in the barley spike during drought stress
Tilahun Abebe, University of Northern Iowa (Tilahun.Abebe@uni.edu)
Experiment design (24 hybridizations)
stress condition
•Control •Drought stress
tissue type name
•Lemma •Palea •Awn •Seed

Barley plants (Hordeum vulgare L. cv. Morex) were grown in a controlled environment chamber at 22°C day/18°C night temperatures, 60% relative hum...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression in the barley spike during drought stress
Accession No: BB89
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
stress condition
•Control   •Drought stress
tissue type name
•Lemma   •Palea   •Awn   •Seed
Quality Control: biological replicates
Treatment summary:
 stress condition  tissue type name  # replicates
 Control  Lemma  3
 Control  Palea  3
 Control  Awn  3
 Control  Seed  3
 Drought stress  Lemma  3
 Drought stress  Palea  3
 Drought stress  Awn  3
 Drought stress  Seed  3
Total hybridizations: 24
Description: Barley plants (Hordeum vulgare L. cv. Morex) were grown in a controlled environment chamber at 22°C day/18°C night temperatures, 60% relative humidity and 16 h light with an intensity of 700 μmoles m-2s-1. Plants were grown in 20 cm x 25 cm pots and fertilized with Osmocote® slow release fertilizer (NPK, 19-6-12). Drought stress was initiated at Zadok stage 71 (kernel watery-ripe; Zadoks et al., 1974) by withholding water for four days. Control (non-stressed) plants received 500 ml water every day. Drought-stressed and control lemma, palea, awn and seed were collected on the fourth day of stress at the grain-filling stage (Zadok scale 83) to compare differences in the response of the transcriptome using a randomized complete block design (RCBD). Drought stress and organ type were the main treatment factors. For each stress level by organ combination, triplicate samples were collected on three different dates (blocks). RNA from each replicate sample was hybridized to individual Barley1 GeneChips. For four organs with two drought stress levels and three biological replications, twenty-four GeneChips were used.
Publication: 'Drought response in the spikes of barley: gene expression in the lemma, palea, awn, and seed.', Tilahun Abebe1, Kalpalatha Melmaiee1, Virginia Berg and Roger P. Wise.
Functional & Integrative Genomics : epub ahead of print (Nov 2009)
Created: 2009-09-29 11:00:43
Last Update: 2009-09-29 12:49:33
GEO Accession GSE17669
Submitter: Tilahun Abebe
Name: Tilahun Abebe
Institution: University of Northern Iowa
Head of Laboratory: Dr. Tilahun Abebe
email(s):
Homepage:

 
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