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Browse CitrusPLEX Experiment Data

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Transcriptional analysis of epithelial cells in grapefruit peel
Siau Sie Voo, Washington State University, Institute of Biological Chemistry, Pullman, Washington (ssvoo@wsu.edu)
Experiment design (12 hybridizations)
cell type name
•epithelial cell •parenchyma cell
fruit stage
•28mm •41mm

This experiment has been imported by PLEXdb from NCBI GEO (GSE33964)

Series_summary:
Epithelial cells (EC) lining the most inner laye...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptional analysis of epithelial cells in grapefruit peel
Accession No: CT10
Microarray: Citrus
Visibility: public
Experiment Type:
Experiment Factor(s):
cell type name
•epithelial cell   •parenchyma cell
fruit stage
•28mm   •41mm
Quality Control: biological replicates
Treatment summary:
 cell type name  fruit stage  # replicates
 epithelial cell  28mm  3
 epithelial cell  41mm  3
 parenchyma cell  28mm  3
 parenchyma cell  41mm  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE33964)

Series_summary:
Epithelial cells (EC) lining the most inner layers of secretory glands in Citrus peel are hypothesized to be the specialized cells that synthesize citrus essential oil. The major biosynthetic pathway(s) for essential oil are therefore postulated to be specifically and highly active in EC; transcripts that are involved in the pathway(s) are expected to be highly up-regulated in the cells as well.
We performed cell-specific transcriptional analysis using GeneChip Citrus Genome Arrays to probe the global gene expression in EC during initial stage of essential oil biosynthesis and to identify groups of highly expressed genes in the EC.

Series_overall_design:
Grapefruits of two different sizes (diameters at 28 mm and 41 mm), at which points where biosynthesis of essential oil entering its active phase, were selected for the purpose of this experiment. For comparison, we selected parenchyma cells (PC), the non-oil-biosynthesizing cells, as the control cell type. Grapefruit peels were fixed, embedded in paraffin, sectioned and mounted on slides prior to cells isolation using laser microdissection and pressure catapulting. RNA was extracted from the isolated cells and then used for microarray hybridization. Transcriptional analysis were carried out by comparing transcript profiles between different cell types of the same time points, and same cell type at the different time points.
Publication: none
Created: 2012-05-17 11:00:00
Last Update: 2012-05-17 13:42:52
Released: 2012-05-23
GEO Accession GSE33964
Submitter: PLEXdb Curator
Name: Siau Sie Voo
Institution: Washington State University, Institute of Biological Chemistry, Pullman, Washington
Head of Laboratory: Bernd Markus Lange
email(s):
Homepage:

 
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