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Browse CitrusPLEX Experiment Data

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Expression data of sweet orange in response to Candidatus Liberibacter asiaticus infection at advanced stage
Fred G. Gmitter, University of Florida, Lake Alfred, Florida (fgmitter@ufl.edu)
Experiment design (6 hybridizations)
pathogen inoculation
•mock •inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE29633)

Series_summary:
Citrus Huanglongbing (HLB, or greening) is one o...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data of sweet orange in response to Candidatus Liberibacter asiaticus infection at advanced stage
Accession No: CT5
Microarray: Citrus
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•mock   •inoculated
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  # replicates
 mock  3
 inoculated  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE29633)

Series_summary:
Citrus Huanglongbing (HLB, or greening) is one of the most severe diseases of citrus. Plant disease symptom development is considered to be the consequence of a number of molecular, cellular and physiological changes, and may also be associated with host defense responses. Understanding citrus host response to HLB may contribute to the development of new strategies to control this destructive disease.
We performed microarray analysis to identify the differentially expressed genes in sweet orange in response to HLB infection using the Affymetrix GeneChip® citrus genome array.

Series_overall_design:
Two-year-old seedlings of ‘Madam Vinous’ sweet orange (Citrus sinensis L. Osbeck) were inoculated by grafting with bud sticks from HLB-diseased, PCR positive sweet orange plants. For mock-inoculated controls, the same types of plants were grafted with bud sticks from HLB-free, PCR negative sweet orange. At 7 months after inoculation, mature leaves were sampled from 3 individual HLB-diseased plants, and healthy leaves from 3 mock-inoculated plants as control. Total RNA was extracted from leaf samples and hybridized on Affymetrix microarrays.
Publication: none
Created: 2011-10-04 11:04:40
Last Update: 2011-10-04 11:54:05
Released: 2011-10-04
GEO Accession GSE29633
Submitter: PLEXdb Curator
Name: Fred G. Gmitter
Institution: University of Florida, Lake Alfred, Florida
Head of Laboratory: Fred G. Gmitter
email(s):
Homepage:

 
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