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Browse CitrusPLEX Experiment Data

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Transcriptional response of susceptible and tolerant citrus to infection with Candidatus Liberibacter asiaticus
Ute Albrecht, USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida (ute.albrecht@ars.usda.gov)
Experiment design (12 hybridizations)
genotype
•Cleopatra •US-897
pathogen inoculation
•control •inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE30502)

Series_summary:
Candidatus Liberibacter asiaticus (Las), a non-c...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptional response of susceptible and tolerant citrus to infection with Candidatus Liberibacter asiaticus
Accession No: CT6
Microarray: Citrus
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Cleopatra   •US-897
pathogen inoculation
•control   •inoculated
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  # replicates
 Cleopatra  control  3
 Cleopatra  inoculated  3
 US-897  control  3
 US-897  inoculated  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE30502)

Series_summary:
Candidatus Liberibacter asiaticus (Las), a non-culturable phloem-limited bacterium, is the suspected causal agent of Huanglongbing (HLB) in Florida. HLB is one of the most devastating diseases of citrus and no resistant cultivars have been identified to date, though tolerance has been suggested in the genus Poncirus. A recent study conducted in our laboratory demonstrated tolerance of US-897, a hybrid of Poncirus trifoliata (L. Raf.) and the susceptible ‘Cleopatra’ mandarin (Citrus reticulata Blanco), to Ca. L. asiaticus, the presumed causal agent of HLB in Florida (Albrecht & Bowman, HortScience 46 (2011) 16-22).
This study compares transcriptional changes in tolerant US-897 and susceptible ‘Cleopatra’ mandarin seedlings in response to infection with Las using the Affymetrix GeneChip citrus array with the main objective of identifying genes associated with tolerance to HLB. Such genes may be suitable as potential targets for biotechnology approaches, providing one strategy to possibly control this destructive disease of citrus.

Series_overall_design:
Fifteen months-old seedlings of the genotypes 'Cleopatra' mandarin and US-897 were graft-inoculated with non-infected (control) or Las-infected tissue from greenhouse-grown 'Valencia' (C. sinensis L.) plants. Six non-infected control plants each from 'Cleopatra' (MC) and US-897 (TC) and six infected plants each from Cleopatra (MI) and US-897 (TI) were used. Four to six leaves per plant were excised at 32 weeks after inoculation (wai) and immediately frozen in liquid nitrogen. Total RNA was extracted from all 24 plants. Equal amounts of RNA from two samples were pooled to obtain three biological replicates per genotype and treatment and used for hybridization on Affymetrix citrus microarrays.
Publication: none
Created: 2011-11-21 11:13:22
Last Update: 2011-11-21 12:06:13
Released: 2011-11-21
GEO Accession GSE30502
Submitter: PLEXdb Curator
Name: Ute Albrecht
Institution: USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida
Head of Laboratory: Ute Albrecht
email(s):
Homepage:

 
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