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Gene expression in Citrus sinensis (L.) Osbeck following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing Huanglongbing in Florida
Ute Albrecht, USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida (ute.albrecht@ars.usda.gov)
Experiment design (10 hybridizations)
pathogen infection
•infected •healthy
time
•5-9 wai •13-17 wai

This experiment has been imported by PLEXdb from NCBI GEO (GSE33459)

Series_summary:
Huanglongbing (HLB) (=citrus greening) is a dest...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression in Citrus sinensis (L.) Osbeck following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing Huanglongbing in Florida
Accession No: CT7
Microarray: Citrus
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen infection
•infected   •healthy
time
•5-9 wai   •13-17 wai
Quality Control: biological replicates
Treatment summary:
 pathogen infection  time  # replicates
 infected  5-9 wai  3
 infected  13-17 wai  3
 healthy  5-9 wai  2
 healthy  13-17 wai  2
Total hybridizations: 10
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE33459)

Series_summary:
Huanglongbing (HLB) (=citrus greening) is a destructive disease of citrus which is caused by a fastidious, phloem-inhabiting bacterium of the genus Candidatus Liberibacter. Large-scale analysis of gene expression changes in ‘Valencia’ orange leaves were studied during the course of 19 weeks after inoculation with Ca. L. asiaticus using the Affymetrix GeneChip® citrus genome array to provide new insights into the molecular basis of citrus response to this pathogen. Of the more than 33,000 probe sets on the microarray 21,067 were expressed in the leaves, of which 279 and 515 were differentially expressed (FDR ≤ 0.05) five to nine and 13-17 weeks after inoculation, respectively. Results from semi-quantitative RT-PCR analysis performed on 14 selected genes were highly correlated with those observed with the microarray. Gene expression changes involved a variety of different processes including cell defense, transport, cellular organization, photosynthesis, and carbohydrate metabolism. Notable was the pathogen-induced accumulation of transcripts for a phloem-specific lectin PP2-like protein. Transcriptional changes and their relation to disease symptom development are discussed. This is the first study of transcriptional profiling in citrus in response to liberibacter infection using microarray technology.
Huanglongbing (HLB) is a destructive disease of citrus which is suspected to be caused by a phloem-inhabiting bacterium of the genus Candidatus Liberibacter. Large-scale analysis of gene expression changes in ‘Valencia’ orange (C. sinensis) leaves were studied during the course of 19 weeks after inoculation with Ca. L. asiaticus (Las), the pathogen associated with HLB in Florida, using the Affymetrix GeneChip® citrus genome array to provide new insights into the molecular basis of citrus response to this pathogen.

Series_overall_design:
Three year-old 'Valencia' orange (C. sinensis) scions on Cleopatra mandarin (C. reticulata Blanco) rootstocks were graft-inoculated with non-infected (control) or Las-infected tissue from greenhouse- (control) or field-grown 'Lisbon' lemon (C. limon) trees. Fully expanded leaves were collected at 5, 9, 13, and 17 weeks after inoculation (wai). Total RNA was extracted from two control plants and from three Las-inoculated plants per time point. Equal amounts of RNA were combined from samples collected 5 wai and 9 wai and from samples collected 13 wai and 17 wai, resulting in two non-infected and three infected biological replicates for both early (5-9 wai) and late (13-17 wai) time points and used for hybridization on Affymetrix citrus microarrays.
Publication: none
Created: 2011-12-13 10:26:35
Last Update: 2011-12-13 12:25:27
Released: 2011-12-13
GEO Accession GSE33459
Submitter: PLEXdb Curator
Name: Ute Albrecht
Institution: USDA, ARS, US Horticultural Research Laboratory, Fort Pierce, Florida
Head of Laboratory: Ute Albrecht
email(s):
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