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Browse CitrusPLEX Experiment Data

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Comparative analysis of stylar canal cells transcriptome in clementine mandarin (Citrus clementina)
Marco Caruso,Stefano La Malfa, University of Catania, Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, Cantania,Italy (mcaruso@unict.it, slamalfa@unict.it)
Experiment design (6 hybridizations)
genotype
•self-incompatible Comune •self compatible Monreal

This experiment has been imported by PLEXdb from NCBI GEO (GSE33014)

Series_summary:
Compared to what is known in model species, repr...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Comparative analysis of stylar canal cells transcriptome in clementine mandarin (Citrus clementina)
Accession No: CT8
Microarray: Citrus
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•self-incompatible Comune   •self compatible Monreal
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 self-incompatible Comune  3
 self compatible Monreal  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE33014)

Series_summary:
Compared to what is known in model species, reproductive biology in citrus is still poorly understood. Although in recent years several efforts have been made to study pollen-pistil interaction and self-incompatibility, little information is available about the molecular mechanisms regulating these processes. We performed microarray analysis for the identification of candidate genes involved in pollen-pistil interaction and self-incompatibility in clementine (Citrus clementina Hort. ex Tan.). The analysis was performed comparing the transcriptome of laser-microdissected stylar canal cells isolated from two clementine genotypes differing for self-incompatibility response (‘Comune’, self-incompatible; and ‘Monreal’, a self compatible mutation of ‘Comune’).

Series_overall_design:
Styles with stigmas, collected 24 hours after self pollination, were immediately snap-frozen in OCT embedding medium (Sakura Finetek, Zoeterwoude, Netherlands) in Peel-A-Way plastic embedding molds (Polysciences, Polysciences, Warrington, PA, USA). Transversal sections 10 µm thick at the upper part of the style were cut with a Leica CM1900 cryostat (Leica Microsystems, Germany) at -20°C. A Leica AS Laser Microdissection system (Leica Microsystems) was used for the isolation of stylar canals from transversal sections. Canals from the stigma were discarded to avoid contamination with pollen or pollen tubes. Three biological replicates were prepared for each genotype. Each biological replicate consisted of bulks of about 200 microdissected areas (composed of an average of 50 cells) coming from two different molds
Publication: 'Comparative transcriptome analysis of stylar canal cells identifies novel candidate genes implicated in the self-incompatibility response of Citrus clementina.', Caruso M, Merelo P, Distefano G, La Malfa S, Lo Piero AR, Tadeo FR, Talon M, Gentile A.
BMC Plant Biol 2012 Feb 14;12:20.
pubmed: 22333138
Created: 2012-04-20 10:29:09
Last Update: 2012-04-24 10:32:01
Released: 2012-04-26
GEO Accession GSE33014
Submitter: PLEXdb Curator
Name: Marco Caruso,Stefano La Malfa
Institution: University of Catania, Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, Cantania,Italy
Head of Laboratory: Alessandra Gentile
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