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Browse CitrusPLEX Experiment Data

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Gene expression analysis in pre- and early post-anthesis stage ovules of Citrus sinensis
Vajinder Kumar, National Research Center on Plant Biotechnology, Department of Biotechnology, New Delhi, India (vgarg_23@yahoo.com)
Experiment design (21 hybridizations)
cultivar
•Vaniglia Sanguigno •Temple
tissue type name
•ovule pre anthesis •ovule post anthesis •ovule inital fruit set stage •leaf

This experiment has been imported by PLEXdb from NCBI GEO (GSE36188)

Series_summary:
Nucellar embryony is a form of apomixis found in...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression analysis in pre- and early post-anthesis stage ovules of Citrus sinensis
Accession No: CT9
Microarray: Citrus
Visibility: public
Experiment Type:
Experiment Factor(s):
cultivar
•Vaniglia Sanguigno   •Temple
tissue type name
•ovule pre anthesis   •ovule post anthesis   •ovule inital fruit set stage   •leaf
Quality Control: technical replicates
Treatment summary:
 cultivar  tissue type name  # replicates
 Vaniglia Sanguigno  ovule pre anthesis  3
 Vaniglia Sanguigno  ovule post anthesis  3
 Vaniglia Sanguigno  ovule inital fruit set stage  3
 Vaniglia Sanguigno  leaf  3
 Temple  ovule pre anthesis  3
 Temple  ovule post anthesis  3
 Temple  ovule inital fruit set stage  3
 Temple  leaf  0
Total hybridizations: 21
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE36188)

Series_summary:
Nucellar embryony is a form of apomixis found in citrus where somatic nucellar cells differentiate into embryos and are included in the seed resulting from the normal sexual process. The nucellar cells giving rise to adventive embryo start proliferating prior to anthesis and fully differentiate obtaining nourishment from sexually derived endosperm. To identify transcripts differentially expressed during nucellar embryo initiation we have taken RNA samplesfrom different developing stages of ovules from polyembryonic (cv. Vaniglia Sanguigno) and monoembryonic (cv. Temple) cultivars. We used microarray for a detailed analysis of global gene expression during nucellar embryony initiation and development. We have further validated the differentially expressed genes using qRT-PCR.

Series_overall_design:
Citrus ovules were harvested from the excised ovaries of different flower developmental stages. These developing stages were decided on the basis of morphological and histological characteristics of flower and female gametophyte, respectively. We selected, Pre anthesis, post anthesis, and initial fruit set stage of flower to excise ovules from Polyembryonic cultivar Vaniglia Sanguigno and Monoembryonic cultivar Temple. Leaf tissue of Vaniglia Sanguigno was used to check the overall transcriptome level differences between leaf and ovule tissues. For each sample, three technical replicates were used.
Publication: none
Created: 2012-04-26 11:44:38
Last Update: 2012-05-01 14:31:58
Released: 2012-05-02
GEO Accession GSE36188
Submitter: PLEXdb Curator
Name: Vajinder Kumar
Institution: National Research Center on Plant Biotechnology, Department of Biotechnology, New Delhi, India
Head of Laboratory: Shripad R. Bhat
email(s):
Homepage:

 
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