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Browse FusariumPLEX Experiment Data

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Fusarium graminearum gene expression during crown rot of wheat
Amber Stephens, Plant Industry CSIRO (amber.stephens@csiro.au)
Experiment design (15 hybridizations)
time
•2 dpi •14 dpi •35 dpi •mycelia cultured

Global Fg gene expression in planta and in vitro was analysed using the Fusarium Affymetrix GeneChip® (Guldener et al., 2006b). For in planta sam...[complete overview]

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Experiment Name: Fusarium graminearum gene expression during crown rot of wheat
Accession No: FG12
Microarray: Fusarium
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•2 dpi  •14 dpi  •35 dpi  •mycelia cultured
Quality Control: biological replicates
Treatment summary:
 time  # replicates
 2 dpi  4
 14 dpi  4
 35 dpi  3
 mycelia cultured  4
Total hybridizations: 15
Description: Global Fg gene expression in planta and in vitro was analysed using the Fusarium Affymetrix GeneChip® (Guldener et al., 2006b). For in planta samples, wheat seedlings were inoculated with Fg at the shoot base and total RNA was obtained at 2, 14 and 35 dpi to correspond with phases 1, 2 and 3 of the infection process, respectively. Each time point consisted of at least three biological replicates taken in parallel and each biological replicate was a pool of 18 stem bases. In order to tentatively identify Fg genes that had been differentially expressed during growth in planta compared to axenic culture conditions, total RNA was extracted from a sample of Fg cultured in complete defined media. This sample consisted of four biological replicates taken in parallel.
Publication: 'Phases of Infection and Gene Expression of Fusarium graminearum During Crown Rot Disease of Wheat', Amber E. Stephens, Donald M. Gardiner, Rosemary G. White, Alan L. Munn and John M. Manners
MPMI
Created: 2008-07-23 22:52:08
Last Update: 2008-07-24 20:34:05
Submitter: Amber Elise Stephens
Name: Amber Stephens
Institution: Plant Industry CSIRO
Head of Laboratory: Dr John Manners
email(s):
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