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Browse FusariumPLEX Experiment Data

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Fusarium graminearum gene expression in wheat stems during infection
Heather Hallen-Adams, Michigan State University (hallenhe@msu.edu,trail@msu.edu)
Experiment design (12 hybridizations)
developmental stage name
•IF - vegetative hyphae •RW - wide dikaryotic hyphae •SW - perithecial initials •YP - young perithecia

Spring wheat cv. "Bobwhite" was inoculated immediately prior to anthesis with 10 ul of a 5x10E5 conidia/ml stock of Fusarium graminearum strain PH-...[complete overview]

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Experiment Name: Fusarium graminearum gene expression in wheat stems during infection
Accession No: FG16
Microarray: Fusarium
Visibility: public
Experiment Type:
Experiment Factor(s):
developmental stage name
•IF - vegetative hyphae   •RW - wide dikaryotic hyphae   •SW - perithecial initials   •YP - young perithecia
Quality Control: biological replicates
Treatment summary:
 developmental stage name  # replicates
 IF - vegetative hyphae  3
 RW - wide dikaryotic hyphae  3
 SW - perithecial initials  3
 YP - young perithecia  3
Total hybridizations: 12
Description: Spring wheat cv. "Bobwhite" was inoculated immediately prior to anthesis with 10 ul of a 5x10E5 conidia/ml stock of Fusarium graminearum strain PH-1. 2 cm samples of stem were collected ~ 15-30 days post-inoculation and sorted into infection front (IF; stem asymptomatic), radial growth (RW; wide binucleate hyphae growing outwards from pith, stem exhibits water-soaking) and senescent wheat (SW; perithecial initials present under stomates, stem appears bleached). Further inoculated plants were allowed to senesce completely in the greenhouse, and stem portions were then harvested and incubated on moist vermiculite to allow perithecium formation (young perithecia; YP).
Publication: 'Triacylglyceride metabolism by Fusarium graminearum during colonization and sexual development on wheat', Guenther, John C, Hallen-Adams, Heather E, Buecking, H, Shachar-Hill, Y, and Trail, F
Molecular Plant Microbe Interactions 22(12):1492-503(2009).
pubmed: 19888815
Created: 2010-04-09 14:53:41
Last Update: 2010-04-15 15:52:46
Released: 2010-04-15
Submitter: Heather E Hallen
Name: Heather Hallen-Adams
Institution: Michigan State University
Head of Laboratory: Frances Trail
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