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Browse SoyPLEX Experiment Data

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Analysis of Iron Deficiency in Soybean Leaf Tissue
Randy Shoemaker, Iowa State University, Department of Agronomy, Ames, Iowa (rcsshoe@iastate.edu)
Experiment design (11 hybridizations)
genotype
•Clark •IsoClark
stress condition
•Iron Sufficient •Iron Defficient

This experiment has been imported by PLEXdb from NCBI GEO (GSE10730) Series_summary: This study was designed to identify candidate genes associ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Analysis of Iron Deficiency in Soybean Leaf Tissue
Accession No: GM26
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Clark   •IsoClark
stress condition
•Iron Sufficient   •Iron Defficient
Quality Control: biological replicates
Treatment summary:
 genotype  stress condition  # replicates
 Clark  Iron Sufficient  3
 Clark  Iron Defficient  2
 IsoClark  Iron Sufficient  3
 IsoClark  Iron Defficient  3
Total hybridizations: 11
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE10730)

Series_summary:
This study was designed to identify candidate genes associated with iron efficiency in soybeans. Two genotypes, Clark (PI548553) and IsoClark (PI547430), were grown in both iron sufficient (100uM Fe(NO3)3) and iron deficient (50uM Fe(NO3)3) hydroponics conditions. The second trifoliate was harvested for RNA extraction for the microarray experiment. Candidate genes were identified by comparing gene expression profiles within genotypes between the two iron growth conditions.
Keywords: Stress Response

Series_overall_design:
This experiment was designed to compare expression profiles of Clark grown in iron sufficient and deficient iron conditions and of IsoClark grown in the same conditions. Plants grown in iron sufficient conditions were used as controls and plants grown in iron deficient conditions were considered experimental. For the Clark genotype, There were two biological replicates of iron deficient plants, and three biological replicates of iron sufficient plants. The IsoClark genotype had three biological replicates for both iron sufficient and deficient conditions.
Publication: 'Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment.', Ge Y, Li Y, Zhu YM, Bai X, Lv DK, Guo D, Ji W, Cai H.
BMC Plant Biol 2010 Jul 26;10:153.
pubmed: 20653984
Created: 2011-04-19 12:50:16
Last Update: 2011-05-17 12:23:38
Released: 2011-05-19
GEO Accession GSE10730
Submitter: PLEXdb Curator
Name: Randy Shoemaker
Institution: Iowa State University, Department of Agronomy, Ames, Iowa
Head of Laboratory: Randy Shoemaker
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