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Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment
Yanming Zhu, Northeast Agriculture University, Department of Life sciences, Harbin, China (ymzhu2001@neau.edu.cn)
Experiment design (14 hybridizations)
time
•0(control) hrs •0.5 hrs •1 hrs •3 hrs •6 hrs •12 hrs •24 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE17883) Series_summary: Plant roots are the primary site of perception and injury ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment
Accession No: GM28
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•0(control) hrs  •0.5 hrs  •1 hrs  •3 hrs  •6 hrs  •12 hrs  •24 hrs
Quality Control: biological replicates
Treatment summary:
 time  # replicates
 0(control) hrs  2
 0.5 hrs  2
 1 hrs  2
 3 hrs  2
 6 hrs  2
 12 hrs  2
 24 hrs  2
Total hybridizations: 14
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17883)

Series_summary:
Plant roots are the primary site of perception and injury for saline-alkaline stress. The current knowledge of the saline-alkaline stress transcriptome is most focused on salt (NaCl) stress. Only a little alkaline (NaHCO3) stress transcriptome is limited to one time point after stress. Time-course analysis and comparative investigation on roots in the alkaline stress condition are needed to understand the gene response networks that are subject to alkaline tolerance.
We used microarrays to detail the global programme of gene expression underlying NaHCO3 treatment and identified distinct classes of regulated genes during this process.

Series_overall_design:
Three week old Glycine soja seedling roots from 3cm root apex were harvested in two independent biological replicates after 0, 0.5, 1, 3, 6, 12 and 24h treatment with 50mmol/L NaHCO3 stress for RNA extraction and hybridization on Affymetrix microarrays. To minimize biological variance, roots from three plants originating from the same experiment, condition and cultivar was pooled.
Publication: 'Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment.', Ge Y, Li Y, Zhu YM, Bai X, Lv DK, Guo D, Ji W, Cai H.
BMC Plant Biol 2010 Jul 26;10:153.
pubmed: 20653984
Created: 2011-04-21 11:53:11
Last Update: 2011-05-31 10:48:57
Released: 2011-05-31
GEO Accession GSE17883
Submitter: PLEXdb Curator
Name: Yanming Zhu
Institution: Northeast Agriculture University, Department of Life sciences, Harbin, China
Head of Laboratory: Yanming Zhu and Dianjing Guo
email(s):
Homepage:

 
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