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Gene expression profiling in soybean under aluminum stress: mechanisms of magnesium amelioration of aluminum toxicity
Dongquan Chen, West Virginia University, Department of Medicine, Morgantown, West Virginia (dchen@hsc.wvu.edu)
Experiment design (24 hybridizations)
genotype
•PI416937 •Young
compound
•Aluminum •Aluminum+Magnesium
time
•12 hrs •72 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE18518) Series_summary: Gene expression profiling in soybean under aluminum stres...[complete overview]

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Experiment Name: Gene expression profiling in soybean under aluminum stress: mechanisms of magnesium amelioration of aluminum toxicity
Accession No: GM29
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•PI416937   •Young
compound
•Aluminum   •Aluminum+Magnesium
time
•12 hrs  •72 hrs
Quality Control: biological replicates
Treatment summary:
 genotype  compound  time  # replicates
 PI416937  Aluminum  12 hrs  3
 PI416937  Aluminum  72 hrs  3
 PI416937  Aluminum+Magnesium  12 hrs  3
 PI416937  Aluminum+Magnesium  72 hrs  3
 Young  Aluminum  12 hrs  3
 Young  Aluminum  72 hrs  3
 Young  Aluminum+Magnesium  12 hrs  3
 Young  Aluminum+Magnesium  72 hrs  3
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18518)

Series_summary:
Gene expression profiling in soybean under aluminum stress: mechanisms of magnesium amelioration of aluminum toxicity at gene expression level.
Micro-molar concentrations of magnesium in culture solution has been shown to ameliorate Al toxicity in soybean and other leguminous species. Different theories have been proposed to explain the chemical mechanisms of how the two ions interact to neutralize the toxic effect of aluminum in the plant root system. To understand the molecular mechanisms of the phenomenon at the gene expression level in soybean, we undertook a comparative transcriptome analysis in Al-tolerant and Al-sensitive genotypes treated with aluminum alone or aluminum plus magnesium using DNA microarrays. The results revealed magnesium enhances Al-tolerance level in the Al-tolerant genotype by down-regulating genes commonly induced in response to Al toxicity. We hypothesized that the magnesium-mediated alleviation of Al toxicity in the Al-tolerant genotype emanates from reduction in energy expenditure of gene expression induced in response to Al stress. Conversely, magnesium appears to ameliorate Al toxicity in the sensitive genotype by dual mechanisms of increasing the expression level of several genes involved in Al-tolerance and decreasing the expression level of most genes.
Keywords: Soybean, aluminum toxicity, magnesium, transcriptome

Series_overall_design:
Two genotypes: PI 416937 (p) and Young (y); two treatments: Aluminum (Al) or Al+magnesium (Mg); two time points: 12 and 72 hrs; 3 replicates.
Publication: 'Mechanisms of magnesium amelioration of aluminum toxicity in soybean at the gene expression level.', Duressa D, Soliman KM, Chen D.
Genome 2010 Oct;53(10):787-97.
pubmed: 20962885
Created: 2011-04-21 12:42:57
Last Update: 2011-06-02 11:27:51
Released: 2011-06-02
GEO Accession GSE18518
Submitter: PLEXdb Curator
Name: Dongquan Chen
Institution: West Virginia University, Department of Medicine, Morgantown, West Virginia
Head of Laboratory: Khairy M. Soliman
email(s):
Homepage:

 
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