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Circadian clock controlled gene expression in soybean seeds
Karen A.Hudson, USDA-ARS, Department of Crop Production and Pest Control, West Lafayette, Indiana (karen.hudson@ars.usda.gov)
Experiment design (26 hybridizations)
time
•24 hrs •28 hrs •32 hrs •36 hrs •40 hrs •44 hrs •48 hrs •52 hrs •56 hrs •60 hrs •64 hrs •68 hrs •72 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE18827) Series_summary: Microarray expression profiling was used to identify gen...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Circadian clock controlled gene expression in soybean seeds
Accession No: GM31
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•24 hrs  •28 hrs  •32 hrs  •36 hrs  •40 hrs  •44 hrs  •48 hrs  •52 hrs  •56 hrs  •60 hrs  •64 hrs  •68 hrs  •72 hrs
Quality Control: biological replicates
Treatment summary:
 time  # replicates
 24 hrs  2
 28 hrs  2
 32 hrs  2
 36 hrs  2
 40 hrs  2
 44 hrs  2
 48 hrs  2
 52 hrs  2
 56 hrs  2
 60 hrs  2
 64 hrs  2
 68 hrs  2
 72 hrs  2
Total hybridizations: 26
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18827)

Series_summary:
Microarray expression profiling was used to identify genes expressed in developing soybean (Glycine max) seeds that are controlled by the circadian clock. Plants with developing seeds were entrained to 12hour light: 12 hour dark cycles and sampled in constant light conditions.

Series_overall_design:
Soybean seeds entrained to 12 hour-light:12 hour dark photocycles were harvested after 24 hours of constant light and temperature conditions. Timepoint 24h represents subjective dawn. To minimize developmental variation, flowers were marked as they opened and seeds were harvested from marked pods after 5 weeks. There are two replicate samples at each time point, and each sample consists of 8 seeds collected from three diffferent plants.
Publication: 'The Circadian Clock-controlled Transcriptome of Developing Soybean Seeds.', Karen A. Hudson
The Plant Genome, Jul 2010; 3: 3 - 13. doi: 10.3835/?plantgenome2009.08.0025
Created: 2011-04-22 13:20:45
Last Update: 2011-06-03 15:01:37
Released: 2011-06-03
GEO Accession GSE18827
Submitter: PLEXdb Curator
Name: Karen A.Hudson
Institution: USDA-ARS, Department of Crop Production and Pest Control, West Lafayette, Indiana
Head of Laboratory: Karen A Hudson
email(s):
Homepage:

 
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