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Alterations in soybean gene expression profile after foliar application of lipo-chitooligosaccharide (LCO) from Bradyrhizobium japonicum under sub-optimal temperature
Nan Wang, McGill University/MacDonald Campus, Department of Dept. of Plant Science, Ste-Anne-de-Bellevue, Canada (not-in-geo)
Experiment design (12 hybridizations)
compound
•control •LCO
time
•0 hrs •48 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE20972) Series_summary: Lipo-chitooligosaccharides (LCOs) produced by N2-fixing r...[complete overview]

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Experiment Name: Alterations in soybean gene expression profile after foliar application of lipo-chitooligosaccharide (LCO) from Bradyrhizobium japonicum under sub-optimal temperature
Accession No: GM33
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•control   •LCO
time
•0 hrs  •48 hrs
Quality Control: biological replicates
Treatment summary:
 compound  time  # replicates
 control  0 hrs  3
 control  48 hrs  3
 LCO  0 hrs  3
 LCO  48 hrs  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE20972)

Series_summary:
Lipo-chitooligosaccharides (LCOs) produced by N2-fixing rhizobacteria initiate host nodule formation. Foliar application of LCOs has been shown to induce stress-related genes under optimal growth conditions. To study the effects of LCO foliar spray under stressed conditions, soybean seedlings grown at optimal temperature were exposed to sub-optimal temperature. After a 5-day acclimation period, the first trifoliolate leaves were sprayed with 10-7 M LCO (NodBj-V (C18:1, MeFuc)) produced by Bradyrhizobium japonicum, and harvested at 0 and 48 h following treatment. Microarray analysis was performed using Affymetrix GeneChip® Soybean Genome Arrays. A total of 147 genes were differentially expressed 48 h after LCO treatment, including a number of stress-related genes and transcription factors. In addition, during the 48 h following treatment, hundreds of genes were differentially expressed in LCO-treated plants, indicating that the dynamic soybean foliar transcriptome was highly responsive to LCO treatment. The microarray data was supported by quantitative real-time PCR data.

Series_overall_design:
Soybean seedlings grown at optimal temperature (25 °C) were exposed to sub-optimal temperature (15 °C). After a 5-day acclimation period, the first trifoliolate leaves were sprayed with 10-7 M LCO (NodBj-V (C18:1, MeFuc)) produced by Bradyrhizobium japonicum, and harvested at 0 and 48 h following treatment. Total RNA was extracted and microarray analysis was performed using Affymetrix GeneChip® Soybean Genome Arrays.
Publication: none
Created: 2011-04-26 13:30:03
Last Update: 2011-06-07 10:39:04
Released: 2011-06-07
GEO Accession GSE20972
Submitter: PLEXdb Curator
Name: Nan Wang
Institution: McGill University/MacDonald Campus, Department of Dept. of Plant Science, Ste-Anne-de-Bellevue, Canada
Head of Laboratory: Donald L. Smith
email(s):
Homepage:

 
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