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Expression of the Stress-Related Genes for Glutathione S-Transferase and Ascorbate Peroxidase in the Most-Glycinin-Deficient Soybean Cultivar Tousan205 during Seed Maturation.
Tomoyo Narikawa, University of Tokyo, Department of Taste Science, Tokyo, Japan (asakura@cc.tuat.ac.jp,asakura@mail.ecc.u-tokyo.ac.jp)
Experiment design (8 hybridizations)
cultivar
•Nanahomare •Tamahomare
developmental stage name
•Bean5mm •Full Grown

This experiment has been imported by PLEXdb from NCBI GEO (GSE22158) Series_summary: Global analyses on gene expression profiles in two soybea...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression of the Stress-Related Genes for Glutathione S-Transferase and Ascorbate Peroxidase in the Most-Glycinin-Deficient Soybean Cultivar Tousan205 during Seed Maturation.
Accession No: GM34
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
cultivar
•Nanahomare   •Tamahomare
developmental stage name
•Bean5mm   •Full Grown
Quality Control: biological replicates
Treatment summary:
 cultivar  developmental stage name  # replicates
 Nanahomare  Bean5mm  2
 Nanahomare  Full Grown  2
 Tamahomare  Bean5mm  2
 Tamahomare  Full Grown  2
Total hybridizations: 8
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22158)

Series_summary:
Global analyses on gene expression profiles in two soybean species, Nanahomare and Tamahomare was performed using DNA microarray technique. Nanahomare is glycinin deficient species, and is high in free amino acid content. Tamahomare is parent species of Nanahomare.
In Nanahomare, stress related genes glutathione S-transferase and ascorbate peroxidase had higher expression than in Tamahomare, which suggests glycinin-deficiency caused stress in soy seeds, and that it lead Nanahomare to increase in free amino acid content.
Keywords: storage protein deficiency

Series_overall_design:
Soybean seeds were selected at two stages of Bean 5mm and Full-size for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain difference in gene expression between two species and find out which gene's or gene group's expression changes.
Publication: 'Expression of the stress-related genes for glutathione S-transferase and ascorbate peroxidase in the most-glycinin-deficient soybean cultivar Tousan205 during seed maturation.', Narikawa T, Tamura T, Yagasaki K, Terauchi K, Sanmiya K, Ishimaru Y, Abe K, Asakura T.
Biosci Biotechnol Biochem 2010 Sep 23;74(9):1976-9.
pubmed: 20834138
Created: 2011-04-28 11:07:26
Last Update: 2011-06-09 10:12:09
Released: 2011-06-09
GEO Accession GSE22158
Submitter: PLEXdb Curator
Name: Tomoyo Narikawa
Institution: University of Tokyo, Department of Taste Science, Tokyo, Japan
Head of Laboratory: Tomoyo Narikawa
email(s):
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