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Experiment Name:

Expression of the Stress-Related Genes for Glutathione S-Transferase and Ascorbate Peroxidase in the Most-Glycinin-Deficient Soybean Cultivar Tousan205 during Seed Maturation.

Accession No:

GM34

Microarray:

Soybean

Visibility:

public

Experiment Type:

Experiment Factor(s):

cultivar: •Nanahomare •Tamahomare
developmental stage name: •Bean5mm •Full Grown

Quality Control:

biological replicates

Treatment summary:

cultivar	developmental stage name	# replicates
Nanahomare	Bean5mm	2
Nanahomare	Full Grown	2
Tamahomare	Bean5mm	2
Tamahomare	Full Grown	2

Total hybridizations: 8

Description:

This experiment has been imported by PLEXdb from NCBI GEO (GSE22158)

Series_summary:
Global analyses on gene expression profiles in two soybean species, Nanahomare and Tamahomare was performed using DNA microarray technique. Nanahomare is glycinin deficient species, and is high in free amino acid content. Tamahomare is parent species of Nanahomare.
In Nanahomare, stress related genes glutathione S-transferase and ascorbate peroxidase had higher expression than in Tamahomare, which suggests glycinin-deficiency caused stress in soy seeds, and that it lead Nanahomare to increase in free amino acid content.
Keywords: storage protein deficiency

Series_overall_design:
Soybean seeds were selected at two stages of Bean 5mm and Full-size for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain difference in gene expression between two species and find out which gene's or gene group's expression changes.

Publication:

'Expression of the stress-related genes for glutathione S-transferase and ascorbate peroxidase in the most-glycinin-deficient soybean cultivar Tousan205 during seed maturation.', Narikawa T, Tamura T, Yagasaki K, Terauchi K, Sanmiya K, Ishimaru Y, Abe K, Asakura T.
Biosci Biotechnol Biochem 2010 Sep 23;74(9):1976-9.
pubmed: 20834138

Created:

2011-04-28 11:07:26

Last Update:

2011-06-09 10:12:09

Released:

2011-06-09

GEO Accession

GSE22158

Submitter:

PLEXdb Curator

Experimenter:

Name:	Tomoyo Narikawa
Institution:	University of Tokyo, Department of Taste Science, Tokyo, Japan
Head of Laboratory:	Tomoyo Narikawa
email(s):	asakura@cc.tuat.ac.jp,asakura@mail.ecc.u-tokyo.ac.jp,asakura@cc.tuat.ac.jp,asakura@mail.ecc.u-tokyo.ac.jp
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