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Expression data of Soybean (Glycine max) roots from different iron treatments.
Carroll P. Vance, USDA-ARS, University of Minnesota, Department of Agronomy and Plant Genetics, St. Paul, Minnesota (vance004@umn.edu)
Experiment design (24 hybridizations)
genetic line
•IsoClark •Clark
stress condition
•Fe limited •Fe sufficient •Fe recovery •Fe sustained

This experiment has been imported by PLEXdb from NCBI GEO (GSE22227) Series_summary: Two Near Isogenic soybean (Glycine max) lines were grown i...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data of Soybean (Glycine max) roots from different iron treatments.
Accession No: GM35
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•IsoClark   •Clark
stress condition
•Fe limited   •Fe sufficient   •Fe recovery   •Fe sustained
Quality Control: biological replicates
Treatment summary:
 genetic line  stress condition  # replicates
 IsoClark  Fe limited  3
 IsoClark  Fe sufficient  3
 IsoClark  Fe recovery  3
 IsoClark  Fe sustained  3
 Clark  Fe limited  3
 Clark  Fe sufficient  3
 Clark  Fe recovery  3
 Clark  Fe sustained  3
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22227)

Series_summary:
Two Near Isogenic soybean (Glycine max) lines were grown in hydroponic conditions with either 50uM ferric nitrate or 100uM ferric nitrate. After 10 days, half the plants were harvested (total root tissue). At 12 days after planting, iron was added to plants grown in low iron conditions bringing them up to sufficient iron growth conditions. Root tissue was harvested for the remaining plants at 14 days after planting.
Gene expression analysis from root tissue of two Near Isogenic Lines (NILs), Clark (PI548553) and IsoClark (PI547430), grown in iron stress or iron stress recovered conditions.

Series_overall_design:
A total of 24 samples from four growth conditions, three biological replicates per treatment.
Publication: 'RNA profiling of fusarium head blight-resistant wheat addition lines containing the Thinopyrum elongatum chromosome 7E.', Wang, J. -R., Wang, L., Gulden, S., Rocheleau, H., Balcerzak, M., Hattori, J., Cao, W., Han, F., Zheng, Y. -L., Fedak, G. and Ouellet, T.
Canadian Journal of Plant Pathology, Volume 32, Issue 2 April 2010, pages 188 - 214. DOI: 10.1080/07060661003740512
Created: 2011-04-28 11:54:31
Last Update: 2011-06-10 15:06:01
Released: 2011-06-10
GEO Accession GSE22227
Submitter: PLEXdb Curator
Experimenter:
Name: Carroll P. Vance
Institution: USDA-ARS, University of Minnesota, Department of Agronomy and Plant Genetics, St. Paul, Minnesota
Head of Laboratory: Robert M. Stupar
email(s):
vance004@umn.edu,rstupar@umn.edu
Homepage:

 
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