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Browse SoyPLEX Experiment Data

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Molecular characterization of the incompatible and compatible interaction of soybean rust disease in Rpp3-containing PI462312 line
Martijn van de Mortel (for Steve Whitham), Iowa State University - Dept Plant Pathology (swhitham@iastate.edu)
Experiment design (54 hybridizations)
pathogen isolates
•Mock •Taiwan 80-2 (vir) •Hawaii 94-1 (avir)
time
•12 hai •24 hai •72 hai •144 hai •216 hai •288 hai

Experimental design: 1 genotype: PI- (resistant USDA Plant Introduction (PI462312) line containing SBR Rpp3 resistance gene) 3 treatments: Virule...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Molecular characterization of the incompatible and compatible interaction of soybean rust disease in Rpp3-containing PI462312 line
Accession No: GM36
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen isolates
•Mock   •Taiwan 80-2 (vir)   •Hawaii 94-1 (avir)
time
•12 hai  •24 hai  •72 hai  •144 hai  •216 hai  •288 hai
Quality Control: biological replicates
Treatment summary:
 pathogen isolates  time  # replicates
 Mock  12 hai  3
 Mock  24 hai  3
 Mock  72 hai  3
 Mock  144 hai  3
 Mock  216 hai  3
 Mock  288 hai  3
 Taiwan 80-2 (vir)  12 hai  3
 Taiwan 80-2 (vir)  24 hai  3
 Taiwan 80-2 (vir)  72 hai  3
 Taiwan 80-2 (vir)  144 hai  3
 Taiwan 80-2 (vir)  216 hai  3
 Taiwan 80-2 (vir)  288 hai  3
 Hawaii 94-1 (avir)  12 hai  3
 Hawaii 94-1 (avir)  24 hai  3
 Hawaii 94-1 (avir)  72 hai  3
 Hawaii 94-1 (avir)  144 hai  3
 Hawaii 94-1 (avir)  216 hai  3
 Hawaii 94-1 (avir)  288 hai  3
Total hybridizations: 54
Description: Experimental design:
1 genotype: PI- (resistant USDA Plant Introduction (PI462312) line containing SBR Rpp3 resistance gene)
3 treatments: Virulent soybean rust (Phakopsora pachyrhizi Tw80-2) challenge, avirulent soybean rust challenge (Hw94-1) & mock infection
3 replications
6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation
TOTAL: 54 Affymetrix GeneChip(R) Soybean Genome Arrays

Mock treatment: 0.01% Tween 20
Hawaii 94 treatment: 500,000 spores per ml in 0.01% Tween 20
Taiwan 80-2 treatment: 500,000 spores per ml in 0.01% Tween 20

Submitted at NCBI GEO via PLEXdb.

Publication: 'Biphasic gene expression changes elicited by Phakopsora pachyrhizi in soybean correlate with fungal penetration and haustoria formation', Schneider KT, van de Mortel M, Bancroft TJ, Braun E, Nettleton D, Nelson RT, Frederick RD, Baum TJ, Graham MA, Whitham SA.
Plant Physiol. 2011 Sep;157(1):355-71.
pubmed: 21791600
Created: 2008-01-08 09:43:31
Last Update: 2011-05-18 16:23:14
Released: 2012-10-10
GEO Accession GSE29740
Submitter: Steve Whitham
Name: Martijn van de Mortel (for Steve Whitham)
Institution: Iowa State University - Dept Plant Pathology
Head of Laboratory: Whitham / Baum
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