Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse SoyPLEX Experiment Data

Choose an experiment:
Molecular characterization of the Rpp4 resistance response
Steve Whitham, Iowa State University (swhitham@iastate.edu)
Experiment design (72 hybridizations)
genotype
•Williams •PI459025B
pathogen inoculation
•Mock •Hawaii 94-1
time
•12 hai •24 hai •72 hai •144 hai •216 hai •288 hai

Experimental design: 2 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) & Cultivar Williams...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Molecular characterization of the Rpp4 resistance response
Accession No: GM37
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Williams   •PI459025B
pathogen inoculation
•Mock   •Hawaii 94-1
time
•12 hai  •24 hai  •72 hai  •144 hai  •216 hai  •288 hai
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  time  # replicates
 Williams  Mock  12 hai  3
 Williams  Mock  24 hai  3
 Williams  Mock  72 hai  3
 Williams  Mock  144 hai  3
 Williams  Mock  216 hai  3
 Williams  Mock  288 hai  3
 Williams  Hawaii 94-1  12 hai  3
 Williams  Hawaii 94-1  24 hai  3
 Williams  Hawaii 94-1  72 hai  3
 Williams  Hawaii 94-1  144 hai  3
 Williams  Hawaii 94-1  216 hai  3
 Williams  Hawaii 94-1  288 hai  3
 PI459025B  Mock  12 hai  3
 PI459025B  Mock  24 hai  3
 PI459025B  Mock  72 hai  3
 PI459025B  Mock  144 hai  3
 PI459025B  Mock  216 hai  3
 PI459025B  Mock  288 hai  3
 PI459025B  Hawaii 94-1  12 hai  3
 PI459025B  Hawaii 94-1  24 hai  3
 PI459025B  Hawaii 94-1  72 hai  3
 PI459025B  Hawaii 94-1  144 hai  3
 PI459025B  Hawaii 94-1  216 hai  3
 PI459025B  Hawaii 94-1  288 hai  3
Total hybridizations: 72
Description: Experimental design:
2 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) & Cultivar Williams that does not have a known SBR resistance gene
2 treatments: Soybean rust (Phakopsora pachyrhizi) isolate Hawaii 94-1 & mock infection
3 replications
6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation
TOTAL: 72 Affymetrix GeneChip(R) Soybean Genome Arrays

Mock treatment: 0.01% Tween 20
Hawaii 94-1 treatment: 500,000 spores per ml in 0.01% Tween 20

Submitted at NCBI GEO via PLEXdb.

Publication: none
Created: 2011-05-16 11:38:59
Last Update: 2011-05-18 19:43:06
Released: 2012-10-10
GEO Accession GSE29741
Submitter: Steve Whitham
Name: Steve Whitham
Institution: Iowa State University
Head of Laboratory: Steve Whitham
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters