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Soybean root hair cell response to Bradyrhizobium japonicum inoculation
Marc Libault, University of Missouri, Department of Plant Sciences, Columbia, Missouri (libaultm@missouri.edu)
Experiment design (42 hybridizations)
pathogen inoculation
•B. japonicum •Mock
time
•6 hai •12 hai •18 hai •24 hai •36 hai •48 hai

This experiment has been imported by PLEXdb from NCBI GEO (GSE24145)

Series_summary:
Soybean root hair transcriptional response to th...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Soybean root hair cell response to Bradyrhizobium japonicum inoculation
Accession No: GM38
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•B. japonicum   •Mock
time
•6 hai  •12 hai  •18 hai  •24 hai  •36 hai  •48 hai
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  time  # replicates
 B. japonicum  6 hai  4
 B. japonicum  12 hai  4
 B. japonicum  18 hai  4
 B. japonicum  24 hai  3
 B. japonicum  36 hai  3
 B. japonicum  48 hai  3
 Mock  6 hai  4
 Mock  12 hai  4
 Mock  18 hai  4
 Mock  24 hai  3
 Mock  36 hai  3
 Mock  48 hai  3
Total hybridizations: 42
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE24145)

Series_summary:
Soybean root hair transcriptional response to their inoculation by the symbiotic bacteria B. japonicum involved in soybean nodulation. We used the first generation of an Affymetrix microarray to quantify the abundance of the transcripts from soybean root hair cells inoculated and mock-inoculated by B. japonicum. This experiment was performed on a time-course from 6 to 48 hours after inoculation.

Series_overall_design:
Soybean seeds were sowed on sterile agar medium and grown for 3 days in a growth chamber before being treated with H2O (mock-inoculated) or B. japonicum (inoculated). Soybean root hair cells were isolated at different time points (6hr, 12hr, 18hr, 24hr, 36hr, 48hr) after treatment. For each time point and condition, 3 or 4 independent biological replicates were produced.
Publication: 'Complete transcriptome of the soybean root hair cell, a single-cell model, and its alteration in response to Bradyrhizobium japonicum infection.', Libault M, Farmer A, Brechenmacher L, Drnevich J, Langley RJ, Bilgin DD, Radwan O, Neece DJ, Clough SJ, May GD, Stacey G.
Plant Physiol 2010 Feb;152(2):541-52.
pubmed: 19933387
Created: 2011-05-19 10:17:24
Last Update: 2011-06-21 13:07:12
Released: 2011-06-21
GEO Accession GSE24145
Submitter: PLEXdb Curator
Name: Marc Libault
Institution: University of Missouri, Department of Plant Sciences, Columbia, Missouri
Head of Laboratory: Gary Stacey
email(s):
Homepage:

 
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