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Browse SoyPLEX Experiment Data

Choose an experiment:
Response of Rpp4-silenced plants to soybean rust
Michelle Graham, USDA (michelle.graham@ars.usda.gov)
Experiment design (5 hybridizations)
treatment
•BPMV-LRR •BPMV empty vector

Experimental design: 1 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) 2 treatments: Ru...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Response of Rpp4-silenced plants to soybean rust
Accession No: GM51
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•BPMV-LRR   •BPMV empty vector
Quality Control: biological replicates
Treatment summary:
 treatment  # replicates
 BPMV-LRR  2
 BPMV empty vector  3
Total hybridizations: 5
Description: Experimental design:
1 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene)

2 treatments: Rub inoculation with BPMV-VIGS construct targeting the LRR region of Rpp4 candidate gene. This BPMV construct and the plant tissue used for microarray was described in Meyer et al. (2009) Plant Physiol. 150:295-307. Control plants were inoculated with empty BPMV vector. At three weeks after BPMV inoculation, all plants were inoculated with Soybean rust (Phakopsora pachyrhizi) isolate LA04-1. LA04-1 is a single spore isolate maintained at the Foreign Disease Weed Science Research Unit.

3 replications
1 time point: 14 days after inoculation

TOTAL: 5 Affymetrix GeneChip(R) Soybean Genome Arrays

LA04-1 treatment: 500,000 spores per ml in 0.01% Tween 20

Submitted at NCBI GEO via PLEXdb.

Publication: none
Created: 2012-10-10 14:44:45
Last Update: 2012-10-11 16:20:31
GEO Accession GSE41724
Submitter: Steve Whitham
Name: Michelle Graham
Institution: USDA
Head of Laboratory: Michelle Graham
email(s):
Homepage:

 
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