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Browse SoyPLEX Experiment Data

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Expression data from salinity treated and control soybean
Yuguang Song, Shandong University, School of Life Science, Jinan, China (syg-0423@163.com)
Experiment design (2 hybridizations)
stress condition
•salinity •control

This experiment has been imported by PLEXdb from NCBI GEO (GSE41125)

Series_summary:
Soybean's productivity is significantly compromi...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from salinity treated and control soybean
Accession No: GM52
Microarray: Soybean
Visibility: public
Experiment Type:
Experiment Factor(s):
stress condition
•salinity   •control
Quality Control: biological replicates
Treatment summary:
 stress condition  # replicates
 salinity  1
 control  1
Total hybridizations: 2
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE41125)

Series_summary:
Soybean's productivity is significantly compromised by soil salinity, but, like most plants, it has evolved a variety of mechanisms to aid its survival under environmental stress. The expression of many plant genes is altered by salinity stress.
We used microarrays to detail the global programme of gene expression and identified distinct up or down-regulated genes between salinity stressed and non stressed soybean

Series_overall_design:
Seedlings of the soybean cultivar Williams 82 were grown in vermiculite under a 16h photoperiod at 25 ºC for 14 days. RNA were isolated from the mock (M0, M1, M3, M6, M12, M24) and salinity treated (S0, S1, S3, S6, S12, S24) seedlings. 0.5 µg RNA that extracted from each time point of the mock and salinity-stressed seedlings were mixed respectively to obtain the mock and salinity-stressed RNA pools, and then they were used to synthesize the cDNA. The cDNA was labeled with biotin, and then hybridized to an Affymetrix soybean Genome Array.
Publication: none
Created: 2012-11-01 12:03:32
Last Update: 2012-11-01 13:03:49
Released: 2012-11-01
GEO Accession GSE41125
Submitter: PLEXdb Curator
Name: Yuguang Song
Institution: Shandong University, School of Life Science, Jinan, China
Head of Laboratory: --
email(s):
Homepage:

 
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