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Browse CottonPLEX Experiment Data

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Affymetrix Cotton Genome array expression data of cotton fiber at different developmental stages from different varieties of Gossypium hirsutum
Samir V Sawant, National Botanical Research Institute, Center for Plant and Molecular Breeding, Lucknow, India (samirsawant@nbri.res.in)
Experiment design (89 hybridizations)
genotype
•JKC 725 •JKC 703 •JKC 777 •JKC 783 •JKC 737
developmental stage name
•0dpa •6dpa •9dpa •12dpa •19dpa •25dpa

This experiment has been imported by PLEXdb from NCBI GEO (GSE36228)

Series_summary:
Cotton fiber were used for the expression analysi...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Affymetrix Cotton Genome array expression data of cotton fiber at different developmental stages from different varieties of Gossypium hirsutum
Accession No: GO6
Microarray: Cotton
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•JKC 725   •JKC 703   •JKC 777   •JKC 783   •JKC 737
developmental stage name
•0dpa   •6dpa   •9dpa   •12dpa   •19dpa   •25dpa
Quality Control: biological replicates
Treatment summary:
 genotype  developmental stage name  # replicates
 JKC 725  0dpa  3
 JKC 725  6dpa  3
 JKC 725  9dpa  3
 JKC 725  12dpa  3
 JKC 725  19dpa  3
 JKC 725  25dpa  3
 JKC 703  0dpa  3
 JKC 703  6dpa  3
 JKC 703  9dpa  3
 JKC 703  12dpa  3
 JKC 703  19dpa  3
 JKC 703  25dpa  3
 JKC 777  0dpa  3
 JKC 777  6dpa  3
 JKC 777  9dpa  3
 JKC 777  12dpa  3
 JKC 777  19dpa  3
 JKC 777  25dpa  3
 JKC 783  0dpa  3
 JKC 783  6dpa  3
 JKC 783  9dpa  3
 JKC 783  12dpa  3
 JKC 783  19dpa  3
 JKC 783  25dpa  2
 JKC 737  0dpa  3
 JKC 737  6dpa  3
 JKC 737  9dpa  3
 JKC 737  12dpa  3
 JKC 737  19dpa  3
 JKC 737  25dpa  3
Total hybridizations: 89
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE36228)

Series_summary:
Cotton fiber were used for the expression analysis at different developmental stages
Affymetrix Cotton Genome array were used for the global profiling of gene expression of cotton fiber at different developmental stages

Series_overall_design:
Six developmental stages (0 DPA, 6DPA,9DPA,12DPA,19DPA and 25DPA) of cotton fiber were selected for gene expression studies. The Gene expression profiling of these developmental stages were comparatively analysed in five different genotypes of Gossypium hirsutum. The genotypes namely JKC 725 and JKC 777 were superior in fiber traits as compared to genotypes JKC 703, JKC 737 and JKC 783. Total RNA were extracted from these varieties at six developmental stages. Biotinylated cRNA were synthesized and hybridized on Affymetrix Cotton Genome array according to manufacturer protocol (Affymetrix).Three biological replicates samples were isolated from each developmental stages from five genotypes.
Publication: none
Created: 2012-05-02 09:48:23
Last Update: 2012-05-02 13:30:18
Released: 2012-05-08
GEO Accession GSE36228
Submitter: PLEXdb Curator
Name: Samir V Sawant
Institution: National Botanical Research Institute, Center for Plant and Molecular Breeding, Lucknow, India
Head of Laboratory: Samir V Sawant
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