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Browse CottonPLEX Experiment Data

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Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress during fibre development stages.
Padmalatha K.V., NRCPB, Department of Biotechnology, New Dehli, India (kv.padmalatha@gmail.com)
Experiment design (24 hybridizations)
stress condition
•control •drought
developmental stage name
•0 dpa •5 dpa •10 dpa •20 dpa

This experiment has been imported by PLEXdb from NCBI GEO (GSE29567)

Series_summary:
Transcriptome analysis in cotton during fibre de...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress during fibre development stages.
Accession No: GO8
Microarray: Cotton
Visibility: public
Experiment Type:
Experiment Factor(s):
stress condition
•control   •drought
developmental stage name
•0 dpa   •5 dpa   •10 dpa   •20 dpa
Quality Control: biological replicates
Treatment summary:
 stress condition  developmental stage name  # replicates
 control  0 dpa  3
 control  5 dpa  3
 control  10 dpa  3
 control  20 dpa  3
 drought  0 dpa  3
 drought  5 dpa  3
 drought  10 dpa  3
 drought  20 dpa  3
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE29567)

Series_summary:
Transcriptome analysis in cotton during fibre development stages.
To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out at fibre development stages (0, 5, 10 and 20 dpa/Days post anthesis). Gossypium hirsutum cv. Bikaneri Nerma was used for the gene expression analysis. Cotton plants were subjected to drought stress at peak flowering stage. Samples were collected when the soil moisture content was 19.5% which is 50% of the normal control plots. Gene expression profiles in drought induced and their respective control samples were analyzed using Affymertix cotton Genechip Genome arrays to study the global changes in the expression of genome.

Series_overall_design:
Total RNA was isolated from 0 dpa, 5 dpa, fibre bearing ovules of 10 dpa, and fibre bearing ovules of 20 dpa. Samples were collected from both drought induced and control plants. Biotin labeled cRNA was hybridized on Affymertix cotton Genechip Genome array following the Affymetrix protocols. Three biological replicates were maintained.

PLEXdb Curator's Note:
Subseries GSE29566 (GO7) and *GSE29567(GO8) are part of the superseries GSE29810 (GO9) at GEO.

Publication: 'Genome-wide transcriptomic analysis of cotton under drought stress reveal significant down-regulation of genes and pathways involved in fibre elongation and up-regulation of defense responsive genes.', Padmalatha KV, Dhandapani G, Kanakachari M, Kumar S, Dass A, Patil DP, Rajamani V, Kumar K, Pathak R, Rawat B, Leelavathi S, Reddy PS, Jain N, Powar KN, Hiremath V, Katageri IS, Reddy MK, Solanke AU, Reddy VS, Kumar PA.
Plant Mol Biol 2012 Feb;78(3):223-46.
pubmed: 22143977
Created: 2012-07-19 11:43:47
Last Update: 2012-07-25 11:15:20
Released: 2012-07-25
GEO Accession GSE29567
Submitter: PLEXdb Curator
Name: Padmalatha K.V.
Institution: NRCPB, Department of Biotechnology, New Dehli, India
Head of Laboratory: Vanga Siva Reddy
email(s):
Homepage:

 
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