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Expression data of fruits in the wildtype and ORR tomato plants
Björn Usadel, Max Planck Institute of Molecular Plant Physiology (usadel@mpimp-golm.mpg.de,asaph.aharoni@weizmann.ac.il)
Experiment design (12 hybridizations)
genotype
•wildtype •homozygous Orr •heterozygous Orr •Orr excision line

This experiment has been imported by PLEXdb from NCBI GEO (GSE22110) Series_summary: An Ac/Ds transposon tagged mutant population was screened...[complete overview]

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Experiment Name: Expression data of fruits in the wildtype and ORR tomato plants
Accession No: LE11
Microarray: tomato10k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•wildtype   •homozygous Orr   •heterozygous Orr   •Orr excision line
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 wildtype  3
 homozygous Orr  3
 heterozygous Orr  3
 Orr excision line  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22110)

Series_summary:
An Ac/Ds transposon tagged mutant population was screened for changes in visible fruit phenotypes. One line showed orange, yellow sectors in the fruit and was named Orange ripening (Orr), its transposase free offspring showed Mendelian segregation yielding red, yellow and orange fruit bearing plants in a ratio of 1:2:1. Crossing the an orange fruit plant line to the wild-type yielded only plants bearing yellow fruit. A cross between the yellow fruit bearing progeny yielded 26 plants having red and 17 plants having yellow fruit, suggesting an over-dominant allele. Using inverse PCR analysis showed an insertion in the putative subunit M of the tomato Ndh complex. Subsequently, an Orr Ds transposon excision line was recovered which only showed red pigmented fruit. Here, we describe microarray profiling of tomato fruits from wild-type, heterozygous and homozygous Orr insertion plants and from fruits harvested from the Orr excision line.

Keywords: mutant wild type

Series_overall_design:
Tomato fruits were harvested at breaker stage using wild-type, ORR homozygouse and heterozygous lines as well as Orr excision lines.
To investigate the expression changes in the ORR mutants, wildtype and ORR mutant line fruits were harvested at the breaker stage and subjected to Affymetrix microarray profiling.
Publication: 'An orange ripening mutant links plastid NAD(P)H dehydrogenase complex activity to central and specialized metabolism during tomato fruit maturation.', Nashilevitz S, Melamed-Bessudo C, Izkovich Y, Rogachev I, Osorio S, Itkin M, Adato A, Pankratov I, Hirschberg J, Fernie AR, Wolf S, Usadel B, Levy AA, Rumeau D, Aharoni A.
Plant Cell 2010 Jun;22(6):1977-97.
pubmed: 20571113
Created: 2010-11-09 11:19:46
Last Update: 2010-11-09 11:36:24
Released: 2010-11-09
GEO Accession GSE22110
Submitter: PLEXdb Curator
Name: Björn Usadel
Institution: Max Planck Institute of Molecular Plant Physiology
Head of Laboratory: Asaph Aharoni
email(s):
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