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Genome wide expression analysis of psaA and psbA tobacco mutant plants.
K.V. Padma Latha, NRCPB, Department of Plant Biotechnology, New Delhi, India (kv.padmalatha@gmail.com)
Experiment design (6 hybridizations)
genotype
•psaA mutant •psbA mutant •normal

This experiment has been imported by PLEXdb from NCBI GEO (GSE23626)

Series_summary:
Transcriptome analysis in tobacco mutant plants ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Genome wide expression analysis of psaA and psbA tobacco mutant plants.
Accession No: LE17
Microarray: tomato10k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•psaA mutant   •psbA mutant   •normal
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 psaA mutant  2
 psbA mutant  2
 normal  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE23626)

Series_summary:
Transcriptome analysis in tobacco mutant plants using tomato Genechip Genome array. Tobacco (Nicotiana tabacum cv. Petit Havanna) psaA and psbA deletion mutants were constructed through a targeted deletion of 767 and 1152 nucleotides of coding regions, respectively with two gene cassettes: psbAproR:uidA:psbterR and rrnR:aadA:rbcLterR coding for GUS reporter and spectinomycin selectable marker genes, respectively. Standard established procedures were followed for chloroplast transformation to generate the psaA and psbA deletion mutants based on the homologous recombination. Gene expression profiles in psaA and psbA tobacco mutant plants were analyzed using tomato Genechip Genome array to study the global changes in the expression of genome.

Series_overall_design:
Total RNA was isolated from psaA and psbA tobacco mutant plants along with the wild type plants. Biotin labeled cRNA was hybridized on tomato GeneChip Genome Array following the Affymetrix protocols. Two independent biological replicates were maintained.
Publication: 'Genome-wide transcriptome and proteome analyses of tobacco psaA and psbA deletion mutants.', Leelavathi S, Bhardwaj A, Kumar S, Dass A, Pathak R, Pandey SS, Tripathy BC, Padmalatha KV, Dhandapani G, Kanakachari M, Solanke AU, Kumar PA, Cella R, Siva Reddy V.
Plant Mol Biol 2011 Jul;76(3-5):407-23.
pubmed: 21327516
Created: 2011-09-22 09:00:40
Last Update: 2011-09-22 11:57:58
Released: 2011-09-22
GEO Accession GSE23626
Submitter: PLEXdb Curator
Name: K.V. Padma Latha
Institution: NRCPB, Department of Plant Biotechnology, New Delhi, India
Head of Laboratory: V. Siva Reddy
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