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Molecular analysis of a tomato OGDH antisense line
Marc Lohse, MPIMP-Golm, Potsdam, Germany (lohse@mpimp-golm.mpg.de)
Experiment design (8 hybridizations)
genotype
•OGDH antisense •M82 wildtype

This experiment has been imported by PLEXdb from NCBI GEO (GSE35618)

Series_summary:
Transgenic tomato (Solanum lycopersicum) plants ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Molecular analysis of a tomato OGDH antisense line
Accession No: LE22
Microarray: tomato10k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•OGDH antisense   •M82 wildtype
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 OGDH antisense  4
 M82 wildtype  4
Total hybridizations: 8
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE35618)

Series_summary:
Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the SlSIOGDH gene encoding subunit E1 of the 2-oxoglutarate dehydrogenase protein complex in the antisense orientation and exhibiting considerable reductions in the activity of this enzyme exhibit a considerably reduced rate of respiration. They were, however, characterised by largely unaltered photosynthetic rates and fruit yield but a restricted leaf, stem and root growth rate. The lines displayed markedly altered metabolic profiles including changes in tricarboxylic acid cycle intermediates and in the majority of the amino acids without alterations in pyridine nucleotide content. Moreover, they displayed a generally accelerated development exhibiting early flowering, accelerated fruit ripening and a markedly earlier onset of leaf senescence. In addition, transcript and selective hormone profiling of gibberellins and ABA revealed changes only in the former coupled to changes in transcripts encoding enzymes of gibberellins biosynthesis. The data obtained are discussed in the context of the importance of this enzyme in both photosynthetic and respiratory metabolism as well as in programs of plant development connected to carbon nitrogen interactions.

Series_overall_design:
Three biological replicates per genotype were analysed. Genotypes analyzed were tomato var. M82 wild type plants grown under green house conditions with one 2-oxo-glutarate dehydrogenase antisense line grown under the same conditions.

PLEXdb Curator Notes:
GEO has provided 4 replicates of each although the text mentions 'Three biological replicates per genotype...' under Overall design
Publication: none
Created: 2012-10-30 13:15:02
Last Update: 2012-10-30 13:54:42
Released: 2012-11-01
GEO Accession GSE35618
Submitter: PLEXdb Curator
Name: Marc Lohse
Institution: MPIMP-Golm, Potsdam, Germany
Head of Laboratory: Mark Stitt
email(s):
Homepage:

 
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