Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse TomPLEX Experiment Data

Choose an experiment:
tomato salt response
Wei Sun, Institute of Botany, Chinese Academy of Sciences (sunweicaas@tom.com,sunweicaas@hotmail.com,xjhua@ibcas.ac.cn)
Experiment design (12 hybridizations)
treatment
•unstressed •salt stressed
strain
•tolerant P12365967 •moneymaker

This experiment has been imported by PLEXdb from NCBI GEO (GSE16401) Series_summary: Wild halophytic tomato has long been considered as an ide...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: tomato salt response
Accession No: LE6
Microarray: tomato10k
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•unstressed   •salt stressed
strain
•tolerant P12365967   •moneymaker
Quality Control: biological replicates
Treatment summary:
 treatment  strain  # replicates
 unstressed  tolerant P12365967  3
 unstressed  moneymaker  3
 salt stressed  tolerant P12365967  3
 salt stressed  moneymaker  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE16401)

Series_summary:
Wild halophytic tomato has long been considered as an ideal gene donor for improving salt tolerance in tomato cultivars. Here, a wild tomato genotype, Solanum pimpinellifolium ‘PI365967’ is significantly more salt-tolerant than a cultivar, Solanum lycopersicom ‘moneymaker’. Affymetrix Tomato Genome Arrays was used to compare the transcriptome change of PI365967 and Moneymaker by salt treatment.After treatment with 200 mM NaCl for 5 h, PI365967 showed relatively fewer responsive genes compared to Moneymaker. Salt Overly Sensitive (SOS) pathway was found to be more active in PI365967 than in Moneymaker, coinciding with relatively less accumulation of Na+ in shoots of PI365967. A gene encoding salicylic acid-binding protein 2 (SABP2) was induced by salinity only in PI365967, suggesting a possible role of salicylic acid signaling in salt response of PI365967. The fact that two genes encoding lactoylglutathione lyase were salt-inducible only in PI365967, together with much higher basal expression of several glutathione S-transferase genes, suggested a more effective detoxification system in PI365967.
Key words: salt tolerance, transcriptomic profiling, wild tomato, ion homeostasis, SABP2.

Series_overall_design:
In one treatment, 9 six-leaf-old plants of one tomato genotype (PI365967 or Moneymaker) were divided into three biological replicates and treated hydroponically under sterile condition with (or without) 200 mM NaCl for 5 h. RNA isolated from pooled three individual plants was used in hybridization to one chip, resulting in 12 chips in total.
Publication: 'Comparative transcriptomic profiling of a salt-tolerant wild tomato species and a salt-sensitive tomato cultivar.', Wei Sun, Xinna Xu, Huishan Zhu, Aihua Liu, Lei Liu, Junming Li, and Xuejun Hua.
Plant Cell Physiol 2010 Jun;51(6):997-1006.
pubmed: 20410049
Created: 2010-10-20 13:47:26
Last Update: 2010-10-25 14:08:00
Released: 2010-11-02
GEO Accession GSE16401
Submitter: PLEXdb Curator
Name: Wei Sun
Institution: Institute of Botany, Chinese Academy of Sciences
Head of Laboratory: Xuejun Hua
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters