Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse TomPLEX Experiment Data

Choose an experiment:
Expression data from PSARK::IPT Nicotiana and wildtype plants
Zvi Peleg, University of California, Department of Plant Sciences (zpeleg@ucdavis.edu,eblumwald@ucdavis.edu)
Experiment design (12 hybridizations)
genetic line
•wildtype •transgenic PSARK::IPT
stress condition
•well-watered •water stressed

This experiment has been imported by PLEXdb from NCBI GEO (GSE19787) Series_summary: Stress-induced leaf senescence could be delayed in transg...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Expression data from PSARK::IPT Nicotiana and wildtype plants
Accession No: LE8
Microarray: tomato10k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•wildtype   •transgenic PSARK::IPT
stress condition
•well-watered   •water stressed
Quality Control: biological replicates
Treatment summary:
 genetic line  stress condition  # replicates
 wildtype  well-watered  3
 wildtype  water stressed  3
 transgenic PSARK::IPT  well-watered  3
 transgenic PSARK::IPT  water stressed  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE19787)

Series_summary:
Stress-induced leaf senescence could be delayed in transgenic plants expressing isopentenyltransferase (IPT), an enzyme that catalyzes the rate-limiting step in CK synthesis under the control of SARK, a maturation- and stress-inducible promoter (Rivero et al., 2007). Increased CK production resulted in enhanced drought tolerance of the transgenic PSARK::IPT plants, with minimal yield loss (Rivero et al., 2007). We have used a large-scale expression profiling approach to investigate the effects of PSARK::IPT on gene expression to elucidate the molecular events associated with the CK-induced drought tolerance displayed by the PSARK::IPT plants.
We used tomato genechips to capture the differential transcriptional responses of WT and PSARK::IPT plants under control and water stress conditions

Series_overall_design:
Leaf samples from well watered (1000 mL per day) and water stressed (300 mL per day) plants were collected for RNA extraction and hybridization on Affymetrix microarrays after 45 days of stress.
Publication: none
Created: 2010-10-27 14:35:22
Last Update: 2010-10-27 14:49:22
Released: 2010-11-03
GEO Accession GSE19787
Submitter: PLEXdb Curator
Name: Zvi Peleg
Institution: University of California, Department of Plant Sciences
Head of Laboratory: Eduardo Blumwald
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters