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Transcript profiling of tomato mutants producing anthocyanins in the fruit
Pierdomenico Perata, Sant'Anna School of Advanced Studies, Crop Physiology Center (p.perata@sssup.it)
Experiment design (8 hybridizations)
genotype
•wild type •Aft mutant •atv mutant •Aft atv double mutant

This experiment has been imported by PLEXdb from NCBI GEO (GSE19792) Series_summary: Anthocyanins are high value plant antioxidants which are ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcript profiling of tomato mutants producing anthocyanins in the fruit
Accession No: LE9
Microarray: tomato10k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•wild type   •Aft mutant   •atv mutant   •Aft atv double mutant
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 wild type  2
 Aft mutant  2
 atv mutant  2
 Aft atv double mutant  2
Total hybridizations: 8
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE19792)

Series_summary:
Anthocyanins are high value plant antioxidants which are not present in the fruits of cultivated tomato. However, both the dominant gene Anthocyanin fruit (Aft) and the recessive gene atroviolacea (atv), introgressed into domesticated tomato from two different wild Solanum species, stimulate a limited anthocyanin pigmentation. Surprisingly, double mutant Aft/Aft atv/atv tomatoes are characterised by the presence of anthocyanins in the fruit peel, resulting in intensely purple pigmented fruit.
We carried out a transcript profiling analysis using GeneChip® Tomato Genome Arrays, in order to identify differentially expressed genes when comparing wild type, Aft/Aft, atv/atv, and Aft/Aft atv/atv fruits. The expression pattern of several genes involved in the anthocyanin pathway was analyzed in detail. Among the fruit peel-associated differentially expressed transcripts, genes involved in phenylpropanoid pathway, cell wall composition, biotic and abiotic stress responses, sugar and hormone metabolism were overrepresented in Aft/Aft atv/atv. Transcriptomic analysis thus revealed that the activation of anthocyanin synthesis in tomato fruit was accompanied by a complex remodulation of gene expression, likely affecting important agronomic and merceological traits.

Series_overall_design:
Wild type (Cv. Ailsa Craig, accession number LA2838A), Aft/Aft (accession number LA1996), atv/atv (accession number LA0797) and double mutant (Aft/Aft atv/atv) were grown during the winter season in a controlled heated greenhouse. Fruits were collected at mature green, turning red and red stages of development. The transcriptional profile in Aft/Aft, atv/atv, and Aft/Aft atv/atv fruits when compared to the wild type was analyzed using the GeneChip® Tomato Genome Array.
Publication: none
Created: 2010-11-02 11:25:41
Last Update: 2010-11-02 11:39:59
Released: 2010-11-08
GEO Accession GSE19792
Submitter: PLEXdb Curator
Experimenter:
Name: Pierdomenico Perata
Institution: Sant'Anna School of Advanced Studies, Crop Physiology Center
Head of Laboratory: Pierdomenico Perata
email(s):
p.perata@sssup.it
Homepage:

 
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