Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse MedicagoPLEX Experiment Data

Choose an experiment:
Identification of molecular factors explaining decreased hypocotyl growth at low temperature
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (24 hybridizations)
genotype
•Jemalong A17 •F83005.5
temperature
•10°C •20°C
time
•35°Cd •50°Cd •100°Cd

This experiment has been imported by PLEXdb from NCBI GEO (GSE18449)

Series_summary:
affy_hypoctemp_medicago - The characterization o...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Identification of molecular factors explaining decreased hypocotyl growth at low temperature
Accession No: ME11
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Jemalong A17   •F83005.5
temperature
•10°C   •20°C
time
•35°Cd   •50°Cd   •100°Cd
Quality Control: biological replicates
Treatment summary:
 genotype  temperature  time  # replicates
 Jemalong A17  10°C  35°Cd  2
 Jemalong A17  10°C  50°Cd  2
 Jemalong A17  10°C  100°Cd  2
 Jemalong A17  20°C  35°Cd  2
 Jemalong A17  20°C  50°Cd  2
 Jemalong A17  20°C  100°Cd  2
 F83005.5  10°C  35°Cd  2
 F83005.5  10°C  50°Cd  2
 F83005.5  10°C  100°Cd  2
 F83005.5  20°C  35°Cd  2
 F83005.5  20°C  50°Cd  2
 F83005.5  20°C  100°Cd  2
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18449)

Series_summary:
affy_hypoctemp_medicago - The characterization of several genotypes of the model Legume Medicago truncatula showed a genetic variability for germination and hypocotyl heterotrophic growth at low temperature and optimal temperature. The most important contrast was between the accessions Jemalong A17 and F83005.5. In order to find genes differently expressed between temperatures and genotypes, the present work focuses on transcriptome profiling during hypocotyl heterotrophic growth under low (10°C) and optimal (20°C) temperature conditions for both genotypes. We used Jemalong A17 and F83005.5 seeds coming from the Medicago truncatula Biological Resource Center in Montpellier, produced in 2006. Experiments were performed in the dark to mimic pre-emergence growth. Pots (6.5 cm diameter, 10 cm high) were incubated in growth chambers either at 10°C or 20°C. They were filled with 500g sand and 100ml of a nutrient solution for young seedlings growth (Saglio and Pradet, 1980). Per pot, five scarified seeds were sown at 1.5 cm depth. Seedlings were harvested at three times: 35°Cd (degree day), 50°Cd and 100°Cd. At each timepoint, about 50 seedlings were harvested and hypocotyl lengths was measured. Hypocotyl were cut and immediately frozen in liquid nitrogen for RNA extractions.

Series_overall_design:
Time course of 24 arrays - Medicago
Publication: none
Created: 2011-06-23 09:37:11
Last Update: 2011-06-27 15:10:29
Released: 2011-06-27
GEO Accession GSE18449
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Sandrine Balzergue
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters