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Browse MedicagoPLEX Experiment Data

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Expression data of Medicago truncatula Jemalong A17 roots treated with auxin transport inhibitors
Adriana Parra Rightmyer, Stanford University, Department of Biology, Stanford, California (Adriana@ArtCubeSoftware.com)
Experiment design (18 hybridizations)
growth_regulator
•Buffer •NPA •TIBA
time
•1 days •7 days

This experiment has been imported by PLEXdb from NCBI GEO (GSE27991)

Series_summary:
Rhizobium and allied bacteria form symbiotic nit...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data of Medicago truncatula Jemalong A17 roots treated with auxin transport inhibitors
Accession No: ME15
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
growth_regulator
•Buffer   •NPA   •TIBA
time
•1 days  •7 days
Quality Control: biological replicates
Treatment summary:
 growth_regulator  time  # replicates
 Buffer  1 days  3
 Buffer  7 days  3
 NPA  1 days  3
 NPA  7 days  3
 TIBA  1 days  3
 TIBA  7 days  3
Total hybridizations: 18
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE27991)

Series_summary:
Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. The transcriptional response of M. truncatula roots 1 and 7 days after ATI treatment were opposite to roots treated with S. meliloti.

Series_overall_design:
Three independent biological replicates were performed at each time point (1 and 7 days after treatment) for each treatment (buffer and ATI).


PLEXdb Curator's Note:

1. At GEO this is part of the superseries GSE28174 which contains samples from mixed platforms and hence not importable into PLEXdb. We have only imported this subseries, GSE27991.

2. Although GEO mentions 6 samples in the description; we found there are 18 CEL files. They represent 3 reps for each of the 6 treatments described at GEO and hence we have modified the factorial structure to reflect the 3 reps missing from the descriptions at GEO.
Publication: 'Six nonnodulating plant mutants defective for Nod factor-induced transcriptional changes associated with the legume-rhizobia symbiosis.', Mitra RM, Shaw SL, Long SR.
Proc Natl Acad Sci U S A 2004 Jul 6;101(27):10217-22.
pubmed: 15220482
Created: 2011-08-17 09:20:36
Last Update: 2011-08-30 12:42:25
Released: 2011-08-30
GEO Accession GSE27991
Submitter: PLEXdb Curator
Name: Adriana Parra Rightmyer
Institution: Stanford University, Department of Biology, Stanford, California
Head of Laboratory: Sharon R. Long
email(s):
Homepage:

 
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