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Browse MedicagoPLEX Experiment Data

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Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) and NFP allelic mutants
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (12 hybridizations)
genotype
•nfp-2 •A17
pathogen inoculation
•inoculated •control

This experiment has been imported by PLEXdb from NCBI GEO (GSE20587)

Series_summary:
affy_aphanomyces_medicago - NFP is one of the pu...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) and NFP allelic mutants
Accession No: ME16
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•nfp-2   •A17
pathogen inoculation
•inoculated   •control
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  # replicates
 nfp-2  inoculated  3
 nfp-2  control  3
 A17  inoculated  3
 A17  control  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE20587)

Series_summary:
affy_aphanomyces_medicago - NFP is one of the putative Nod factor receptor and plays therefore a key role in the establishment of symbiosis. nfp allelic mutants are more susceptible to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare the early plant responses, 1 day post inoculation (dpi) between WT and the nfp2 mutant, in order to identify NFP-dependent gene networks during infection.-Fifteen-day-old A17 or nfp-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with a A. euteiches zoospores (105/ml) and harvested one day after inoculation. Three independent repeats were performed.
Keywords: normal vs ems mutant comparison,near isogenic lines (hif)

Series_overall_design:
12 arrays - Medicago
Publication: none
Created: 2011-11-01 09:59:57
Last Update: 2011-11-01 12:26:01
Released: 2011-11-01
GEO Accession GSE20587
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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