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Browse MedicagoPLEX Experiment Data

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Transcriptom analysis of the interaction Medicago truncatula – Ralstonia solanacearum
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (27 hybridizations)
genetic line
•A17 •Sickle •F83005.5
time
•0 hrs •12 hrs •72 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE18473)

Series_summary:
affy_ralstonia_medicago - Ralstonia solanacearum...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptom analysis of the interaction Medicago truncatula – Ralstonia solanacearum
Accession No: ME17
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•A17   •Sickle   •F83005.5
time
•0 hrs  •12 hrs  •72 hrs
Quality Control: biological replicates
Treatment summary:
 genetic line  time  # replicates
 A17  0 hrs  3
 A17  12 hrs  3
 A17  72 hrs  3
 Sickle  0 hrs  3
 Sickle  12 hrs  3
 Sickle  72 hrs  3
 F83005.5  0 hrs  3
 F83005.5  12 hrs  3
 F83005.5  72 hrs  3
Total hybridizations: 27
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18473)

Series_summary:
affy_ralstonia_medicago - Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease. Its infection process was studied with an in vitro inoculation procedure on intact roots of Medicago truncatula. The pathosystem involved susceptible A17 and resistant F83005.5 M truncatula lines infected with the pathogenic strain GMI1000. The mutant A17 line, Sickle, which showed a resistant phenotype was also part of the experiment. To identify host signaling pathway triggered by R. solanacearum infection with a focus on the involvment of ethylene, we used the Medicago Affymetrix array to monitore the expression profiles and the molecular process associated with initial symptoms development (12hpi) and colonization (72hpi). In order to maximize chances to observe differential gene expression, RNA samples were extracted from the root infection zone (root tips) -Three Medicago truncatula lines, A17, F83005.5 and sickle were inoculated with GMI1000 Ralstonai solanacearum strain (107 cfu/ml). RNA were extracted from root extremities (1 cm above the root tip) at time 0, 12h and 72h post inoculation. Three biological repeats were conducted

Series_overall_design:
normal vs disease comparison, time course, 27 arrays - Medicago
Publication: none
Created: 2011-11-01 11:23:47
Last Update: 2011-11-03 13:20:06
Released: 2011-11-03
GEO Accession GSE18473
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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