Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Sugarcane 8k
Experiments
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse MedicagoPLEX Experiment Data

Choose an experiment:
Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.
Helge Küster, Leibniz Universität Hannover, Hannover, Germany (helge.kuester@genetik.uni-hannover.de)
Experiment design (12 hybridizations)
pathogen inoculation
•Gi-Myc •Gm-Myc •un-inoculated
treatment
•20miM-P •2mM-P

This experiment has been imported by PLEXdb from NCBI GEO (GSE32208)

Series_summary:
We used GeneChips to detail the global programme...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.
Accession No: ME18
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•Gi-Myc   •Gm-Myc   •un-inoculated
treatment
•20miM-P   •2mM-P
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  treatment  # replicates
 Gi-Myc  20miM-P  3
 Gi-Myc  2mM-P  0
 Gm-Myc  20miM-P  3
 Gm-Myc  2mM-P  0
 un-inoculated  20miM-P  3
 un-inoculated  2mM-P  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE32208)

Series_summary:
We used GeneChips to detail the global programme of gene expression in response to colonization by arbuscular mycorrhizal fungi and in response to a treatment with phosphate and identified genes differentially expressed during this process.

Series_overall_design:
Medicago truncatula roots were harvested at 28 days post inoculation with the two different arbuscular mycorrhizal fungi Glomus intraradices (Gi-Myc) and Glomus mosseae (Gm-Myc) under low phosphate conditions (20 µM phosphate) or after a 28 days treatment with 2 mM phosphate in the absence of arbuscular mycorrhizal fungi (2mM-P). As a control, uninfected roots grown under low phosphate conditions (20 µM phosphate) were used (20miM-P). Three biological replicates consisting of pools of five roots were used for RNA extraction and hybridization on Affymetrix GeneChips.
Publication: 'Laser-microdissection unravels cell-type specific transcription in arbuscular mycorrhizal roots, including CAAT-box TF gene expression correlating with fungal contact and spread.', Hogekamp C, Arndt D, Pereira P, Becker JD, Hohnjec N, Küster H.
Plant Physiol 2011 Oct 27.
pubmed: 22034628
Created: 2011-11-15 09:16:11
Last Update: 2011-11-15 11:10:36
Released: 2011-11-15
GEO Accession GSE32208
Submitter: PLEXdb Curator
Name: Helge Küster
Institution: Leibniz Universität Hannover, Hannover, Germany
Head of Laboratory: Helge Küster
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters