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Strigolactone biosynthesis requires the symbiotic GRAS-type transcription factors NSP1 and NSP2
Guido Hooiveld, Wageningen University, Department of Division Human Nutrition, Wageningen, Netherlands (guido.hooiveld@wur.nl)
Experiment design (9 hybridizations)
genotype
•Mtnsp1-1 mutant •Mtnsp2-2 mutant •wildtype

This experiment has been imported by PLEXdb from NCBI GEO (GSE26548)

Series_summary:
Legume GRAS-type transcription factors NSP1 and ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Strigolactone biosynthesis requires the symbiotic GRAS-type transcription factors NSP1 and NSP2
Accession No: ME19
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Mtnsp1-1 mutant   •Mtnsp2-2 mutant   •wildtype
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 Mtnsp1-1 mutant  3
 Mtnsp2-2 mutant  3
 wildtype  3
Total hybridizations: 9
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE26548)

Series_summary:
Legume GRAS-type transcription factors NSP1 and NSP2 are essential for Rhizobium Nod factor-induced nodulation. Both proteins are considered to be Nod factor response factors regulating gene expression upon symbiotic signalling. However, legume NSP1 and NSP2 can be functionally replaced by non-legume orthologs; including rice (Oryza sativa) OsNSP1 and OsNSP2. This shows that both proteins are functionally conserved in higher plants, suggesting an ancient function that was conserved during evolution. Here we show that NSP1 and NSP2 are indispensable for strigolactone biosynthesis in the legume Medicago truncatula as well as rice. Mutant nsp1-nsp2 plants hardly produce strigolactones. The lack of strigolactone biosynthesis coincides with strongly reduced DWARF27 expression in both species. Rice and Medicago represent distinct phylogenetic lineages that split ~150 million years ago. Therefore we conclude that regulation of strigolactone biosynthesis by NSP1 and NSP2 is an ancestral function conserved in higher plants. Since strigolactone biosynthesis is highly regulated by environmental conditions like phosphate starvation, NSP1 and NSP2 will be important tools in future studies on the molecular mechanisms by which environmental sensing is translated into regulation of strigolactone biosynthesis. As NSP1 and NSP2 are single copy genes in legumes, it implies that a single protein complex fulfills a dual regulatory function of different downstream targets; symbiotic and non-symbiotic, respectively.

Series_overall_design:
Three biological replications are used for roots of wild type A17, nsp1 and nsp2 mutant plants
Publication: 'Strigolactone Biosynthesis in Medicago truncatula and Rice Requires the Symbiotic GRAS-Type Transcription Factors NSP1 and NSP2.', Liu W, Kohlen W, Lillo A, Op den Camp R, Ivanov S, Hartog M, Limpens E, Jamil M, Smaczniak C, Kaufmann K, Yang WC, Hooiveld GJ, Charnikhova T, Bouwmeester HJ, Bisseling T, Geurts R.
Plant Cell 2011 Oct;23(10):3853-65.
pubmed: 22039214
Created: 2011-12-13 09:34:24
Last Update: 2011-12-13 12:22:08
Released: 2011-12-13
GEO Accession GSE26548
Submitter: PLEXdb Curator
Name: Guido Hooiveld
Institution: Wageningen University, Department of Division Human Nutrition, Wageningen, Netherlands
Head of Laboratory: René Geurts
email(s):
Homepage:

 
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