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Browse MedicagoPLEX Experiment Data

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A transcript profile of Medicago truncatula root meristem cells
Peta,,Holmes, Genomic Interactions Group, Canberra, Australia (peta.holmes@anu.edu.au)
Experiment design (6 hybridizations)
cell type name
•root meristem •Non-meristematic root

This experiment has been imported by PLEXdb from NCBI GEO (GSE8115)

Series_summary: The root apical meristems (RAM) is established durin...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: A transcript profile of Medicago truncatula root meristem cells
Accession No: ME2
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
cell type name
•root meristem   •Non-meristematic root
Quality Control: biological replicates
Treatment summary:
 cell type name  # replicates
 root meristem  3
 Non-meristematic root  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE8115)

Series_summary:
The root apical meristems (RAM) is established during embryogenesis and serves as a source of stem cells for plant growth and organogenesis. Young roots have a simple structure and meristeamtic and non-meristematic cells can be differentiated based on their location. We have used the Affymetrix Medicago Genome Array GeneChip to compare tissue from the root meristem and non-meristematic tissue to identify meristem specific candidate genes based on changes in gene expression before and after differentiation


Keywords: Cell type comparison

Series_overall_design:
Roots were sectioned separating meristematic (root-tip) and non-meristematic root tissue; total RNA was extracted and used for hybridization to Affymetrix arrays
Publication: 'Transcriptional profiling of Medicago truncatula meristematic root cells', Holmes P, Goffard N, Weiller GF, Rolfe BG et al
BMC Plant Biol 2008 Feb 27;8:21
pubmed: 18302802
Created: 2009-07-02 14:16:05
Last Update: 2011-05-24 16:34:45
GEO Accession GSE8115
Submitter: PLEXdb Curator
Name: Peta,,Holmes
Institution: Genomic Interactions Group, Canberra, Australia
Head of Laboratory: --
email(s):
Homepage:

 
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