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Browse MedicagoPLEX Experiment Data

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Gene expression during early stages of arbuscular mycorrhizal root formation in Medicago truncatula.
Helge Kuster, Leibniz Universität Hannover, Hannover, Germany (helge.kuester@genetik.uni-hannover.de)
Experiment design (12 hybridizations)
treatment
•control •Myc
genotype
•A17 •DM13

This experiment has been imported by PLEXdb from NCBI GEO (GSE34617)

Series_summary:
Arbuscular mycorrhiza (AM) interactions between p...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression during early stages of arbuscular mycorrhizal root formation in Medicago truncatula.
Accession No: ME20
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•control   •Myc
genotype
•A17   •DM13
Quality Control: biological replicates
Treatment summary:
 treatment  genotype  # replicates
 control  A17  3
 control  DM13  3
 Myc  A17  3
 Myc  DM13  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE34617)

Series_summary:
Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel gene expression during early stages of Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on mycorrhizal root fragments enriched for early fungal infection stages.
We used Medicago GeneChips to detail the global programme of gene expression in response to early stages of colonization by arbuscular mycorrhizal fungi and identified genes differentially expressed during these early stages.

Series_overall_design:
Medicago truncatula GFP-HDEL hairy roots (genotypes A17 and DMI3) were grown in vertically-oriented petri dishes, incubated at 26°C and inoculated with 8 Gigaspora margarita spores, which were positioned between the lateral roots. G.margarita spores germinated in 2 to 4 days. Hyphopodia were observed after 5-6 days. Root fragments which reacted to the fungal contact were collected and frozen. Non-inoculated control root fragments were harvested at a comparable age.
Publication: 'Plant Genes Related to Gibberellin Biosynthesis and Signaling Are Differentially Regulated during the Early Stages of AM Fungal Interactions.', Ortu G, Balestrini R, Pereira PA, Becker JD, Küster H, Bonfante P.
Mol Plant 2012 Mar 26.
pubmed: 22451647
Created: 2012-05-01 13:19:05
Last Update: 2012-05-01 15:26:05
Released: 2012-05-02
GEO Accession GSE34617
Submitter: PLEXdb Curator
Name: Helge Kuster
Institution: Leibniz Universität Hannover, Hannover, Germany
Head of Laboratory: Paola Bonfante
email(s):
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