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Browse MedicagoPLEX Experiment Data

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Identification of genes involved in the establishment of plant-microorganism interactions
Sandrine Balzergue, INRA, Evry, France (balzerg@evry.inra.fr)
Experiment design (12 hybridizations)
treatment
•zoneII Sin mel •surrounding cells Mel inc •giant cell Mel inc •root Sin mel

This experiment has been imported by PLEXdb from NCBI GEO (GSE19872)

Series_summary:
affy_pathogen_medicago - In compatible interacti...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Identification of genes involved in the establishment of plant-microorganism interactions
Accession No: ME21
Microarray: Medicago61k
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•zoneII Sin mel   •surrounding cells Mel inc   •giant cell Mel inc   •root Sin mel
Quality Control: biological replicates
Treatment summary:
 treatment  # replicates
 zoneII Sin mel  3
 surrounding cells Mel inc  3
 giant cell Mel inc  3
 root Sin mel  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE19872)

Series_summary:
affy_pathogen_medicago - In compatible interaction between plants and biotrophic microorganisms, neoformation of organs occurs to ensure an efficient relationship between both partners. During the interaction between Medicago truncatula and Sinorhizobium meliloti, bacteria induce the development of root nodule with a permanent meristem, and chronically infect plant cells from zone II before differentiating into atmospheric nitrogen fixing bacteroids. M. truncatula is also plant host for root-knot nematodes, such as Meloidogyne incognita. During this compatible pathogenic interaction, root-knot nematodes induce redifferenciation of root cells from the vascular cylinder into specialized feeding cells called “giant cells”. Hyperplasia and hypertrophy of the surrounding cells lead to the formation of typical root galls. This phenomenon invokes host pathways in common with those necessary for nitrogen-nodule formation, suggesting that nematode and rhizobia exploit a relative common strategy of plant cell infection at the cellular and molecular levels. In order to highlight key genes involved in gall and nodule developments, parallel laser microdissection of giant cells from galls and cells from zone II of nodules, followed by transcriptomic analysis, were performed. The RNA pools were extracted from these cells, amplified and used for transcriptomic studies with M. truncatula Affymetrix DNA chips.
Keywords: organ comparison

Series_overall_design:
12 arrays - Medicago infected with M. incognita or S. meliloti.


PLEXdb Curator's Comment:
GEO :: PLEXdb :: Description
N2 :: zoneII Sin mel :: tissue: root nodule infection zone (zone II) , pathogen: Sinorhizobium meliloti
CC :: surrounding cells Mel inc :: tissue: root galls, surrounding cells, pathogen: Meloidogyne incognita
GC :: giant cell Mel inc :: tissue: root galls, giant cells, pathogen: Meloidogyne incognita
Ct :: root Sin mel :: tissue: infected root, pathogen: Sinorhizobium meliloti
Publication: none
Created: 2012-05-08 11:02:42
Last Update: 2012-05-08 13:09:06
Released: 2012-05-10
GEO Accession GSE19872
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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